Selection of Natural and Base-Modified DNA Aptamers for a Camptothecin Derivative.
Curr Protoc Nucleic Acid Chem
; 65: 9.10.1-9.10.19, 2016 06 01.
Article
in En
| MEDLINE
| ID: mdl-27248786
ABSTRACT
Nucleic acid aptamers for small molecules are currently being developed and have a potential role in diverse applications including biosensing, diagnostics, and therapeutics involving low-molecular-weight biomarkers and drugs. To enhance and broaden their functions through chemical modification, systematic evolution of ligands by exponential enrichment (SELEX) selection has been attempted with modified DNA/RNA libraries. Recently, we demonstrated the superior efficacy of base modification for affinity enhancement and the usefulness of unnatural nucleic acid libraries for development of small-molecule aptamers. In this unit, we describe construction of a modified DNA library that includes (E)-5-(2-(N-(2-(N(6) -adeninyl)ethyl))carbamylvinyl)uracil bases and acquisition of high-affinity camptothecin-binding DNA aptamers, in addition to those of the corresponding natural DNA library and aptamers, using the SELEX method. © 2016 by John Wiley & Sons, Inc.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Camptothecin
/
Aptamers, Nucleotide
/
Small Molecule Libraries
Language:
En
Journal:
Curr Protoc Nucleic Acid Chem
Year:
2016
Document type:
Article
Affiliation country: