Cationic liposome-nucleic acid nanoparticle assemblies with applications in gene delivery and gene silencing.
Philos Trans A Math Phys Eng Sci
; 374(2072)2016 Jul 28.
Article
in En
| MEDLINE
| ID: mdl-27298431
ABSTRACT
Cationic liposomes (CLs) are synthetic carriers of nucleic acids in gene delivery and gene silencing therapeutics. The introduction will describe the structures of distinct liquid crystalline phases of CL-nucleic acid complexes, which were revealed in earlier synchrotron small-angle X-ray scattering experiments. When mixed with plasmid DNA, CLs containing lipids with distinct shapes spontaneously undergo topological transitions into self-assembled lamellar, inverse hexagonal, and hexagonal CL-DNA phases. CLs containing cubic phase lipids are observed to readily mix with short interfering RNA (siRNA) molecules creating double gyroid CL-siRNA phases for gene silencing. Custom synthesis of multivalent lipids and a range of novel polyethylene glycol (PEG)-lipids with attached targeting ligands and hydrolysable moieties have led to functionalized equilibrium nanoparticles (NPs) optimized for cell targeting, uptake or endosomal escape. Very recent experiments are described with surface-functionalized PEGylated CL-DNA NPs, including fluorescence microscopy colocalization with members of the Rab family of GTPases, which directly reveal interactions with cell membranes and NP pathways. In vitro optimization of CL-DNA and CL-siRNA NPs with relevant primary cancer cells is expected to impact nucleic acid therapeutics in vivo. This article is part of the themed issue 'Soft interfacial materials from fundamentals to formulation'.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nucleic Acids
/
Transfection
/
Cations
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Gene Silencing
/
Nanoparticles
/
Liposomes
Limits:
Animals
/
Humans
Language:
En
Journal:
Philos Trans A Math Phys Eng Sci
Journal subject:
BIOFISICA
/
ENGENHARIA BIOMEDICA
Year:
2016
Document type:
Article
Affiliation country: