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Crop milk protein is synthesised following activation of the IRS1/Akt/TOR signalling pathway in the domestic pigeon (Columba livia).
Hu, X-C; Gao, C-Q; Wang, X-H; Yan, H-C; Chen, Z-S; Wang, X-Q.
Affiliation
  • Hu XC; a College of Animal Science , South China Agricultural University/ Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture/ South China Collaborative Innovation Center for Poultry Disease Control and Product Safety , Guangzhou , China.
  • Gao CQ; a College of Animal Science , South China Agricultural University/ Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture/ South China Collaborative Innovation Center for Poultry Disease Control and Product Safety , Guangzhou , China.
  • Wang XH; a College of Animal Science , South China Agricultural University/ Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture/ South China Collaborative Innovation Center for Poultry Disease Control and Product Safety , Guangzhou , China.
  • Yan HC; a College of Animal Science , South China Agricultural University/ Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture/ South China Collaborative Innovation Center for Poultry Disease Control and Product Safety , Guangzhou , China.
  • Chen ZS; b Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences , St. John's University , Queens , NY , USA.
  • Wang XQ; a College of Animal Science , South China Agricultural University/ Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture/ South China Collaborative Innovation Center for Poultry Disease Control and Product Safety , Guangzhou , China.
Br Poult Sci ; 57(6): 855-862, 2016 Dec.
Article in En | MEDLINE | ID: mdl-27636164
The experiment was conducted to study whether insulin receptor substance 1 (IRS1) / Protein kinase B (Akt)/target of the rapamycin (TOR) signalling pathway activation stimulates crop milk protein synthesis in the domestic pigeon (Columba livia). Crop milk was collected from ten 1-d-old squabs and analysed for nutrient content. During the non-breeding period and the first day of lactation, blood samples were collected from 5 pairs of breeding pigeons and the levels of prolactin and insulin were determined. Crop samples were collected from 5 pairs of breeders at d 14 and 16 of the incubation period and d 1, 3 and 7 of the lactation period. Crop samples were evaluated for changes in crop weight and thickness and changes in the expression patterns of IRS1/Akt/TOR signalling pathway-related proteins. The results demonstrated that prolactin induces a gradual increase in the relative weight and thickness of the crop, with crops reaching a maximum size at the third day of lactation. Pigeon crop milk contains 64.1% crude protein and 29.7% crude fat based on dry weight. Serum prolactin and insulin levels in the lactation period were significantly higher than those in the non-breeding period. Compared with non-breeding pigeons, the expression of the phosphorylated IRS1 phosphorylated Akt, phosphorylated TOR, phosphorylated ribosomal protein S6 kinase, phosphorylated S6, phosphorylated eukaryotic initiation factor 4E binding protein 1 and eukaryotic initiation factor 4E were significantly up-regulated in the crop of pigeons in the lactation period. In conclusion, prolactin might induce changes in crop tissue and form the physiological structure for crop milk synthesis. Furthermore, the synthesis of crop milk protein is regulated by activation of the IRS1/Akt/TOR signalling pathway.
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Collection: 01-internacional Database: MEDLINE Main subject: Columbidae / Gene Expression Regulation / Avian Proteins Limits: Animals Language: En Journal: Br Poult Sci Year: 2016 Document type: Article Affiliation country: Country of publication:
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Columbidae / Gene Expression Regulation / Avian Proteins Limits: Animals Language: En Journal: Br Poult Sci Year: 2016 Document type: Article Affiliation country: Country of publication: