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Automated, highly reproducible, wide-field, light-based cortical mapping method using a commercial stereo microscope and its applications.
Jiang, Su; Liu, Ya-Feng; Wang, Xiao-Min; Liu, Ke-Fei; Zhang, Ding-Hong; Li, Yi-Ding; Yu, Ai-Ping; Zhang, Xiao-Hui; Zhang, Jia-Yi; Xu, Jian-Guang; Gu, Yu-Dong; Xu, Wen-Dong; Zeng, Shao-Qun.
Affiliation
  • Jiang S; Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China; These authors contributed equally to the study and paper.
  • Liu YF; Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Wuhan, 430074, China; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan,
  • Wang XM; Department of Hand and Upper Extremity Surgery, Jing'an District Central Hospital, Shanghai, 200040, China.
  • Liu KF; Institute of Neuroscience, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
  • Zhang DH; Institute of Neuroscience, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China; Graduate University of Chinese Academy of Sciences, Shanghai 200031, China.
  • Li YD; Institute of Neuroscience, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China; State Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, 100875, China.
  • Yu AP; Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China.
  • Zhang XH; State Key Laboratory of Cognitive Neuroscience and Learning, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, 100875, China.
  • Zhang JY; Institutes of Brain Science, Fudan University, Shanghai, 200031, China; State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center of Brain Science, Fudan University, Shanghai, 200031, China.
  • Xu JG; Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China; Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
  • Gu YD; Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
  • Xu WD; Department of Hand Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, 200040, China; Department of Hand and Upper Extremity Surgery, Jing'an District Central Hospital, Shanghai, 200040, China; State Key Laboratory of Medical Neurobiology, Collaborative Innovation Center
  • Zeng SQ; Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Wuhan, 430074, China; MoE Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan,
Biomed Opt Express ; 7(9): 3478-3490, 2016 Sep 01.
Article in En | MEDLINE | ID: mdl-27699114
We introduce a more flexible optogenetics-based mapping system attached on a stereo microscope, which offers automatic light stimulation to individual regions of interest in the cortex that expresses light-activated channelrhodopsin-2 in vivo. Combining simultaneous recording of electromyography from specific forelimb muscles, we demonstrate that this system offers much better efficiency and precision in mapping distinct domains for controlling limb muscles in the mouse motor cortex. Furthermore, the compact and modular design of the system also yields a simple and flexible implementation to different commercial stereo microscopes, and thus could be widely used among laboratories.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Biomed Opt Express Year: 2016 Document type: Article Country of publication:
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Add to My VHL
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XML
PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Biomed Opt Express Year: 2016 Document type: Article Country of publication: