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Routine identification of Nocardia species by MALDI-TOF mass spectrometry.
Girard, Victoria; Mailler, Sandrine; Polsinelli, Sophie; Jacob, Delphine; Saccomani, Marie Christine; Celliere, Beatrice; Monnin, Valerie; van Belkum, Alex; Hagen, Ferry; Meis, Jacques F; Durand, Géraldine.
Affiliation
  • Girard V; R&D Microbiology, bioMérieux, La Balme Les Grottes, France. Electronic address: victoria.girard@biomerieux.com.
  • Mailler S; R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
  • Polsinelli S; R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
  • Jacob D; R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
  • Saccomani MC; R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
  • Celliere B; R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
  • Monnin V; R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
  • van Belkum A; R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
  • Hagen F; Canisius-Wilhelmina Hospital, Department of Medical Microbiology and Infectious Diseases, Nijmegen, The Netherlands.
  • Meis JF; Canisius-Wilhelmina Hospital, Department of Medical Microbiology and Infectious Diseases, Nijmegen, The Netherlands; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
  • Durand G; R&D Microbiology, bioMérieux, La Balme Les Grottes, France.
Diagn Microbiol Infect Dis ; 87(1): 7-10, 2017 Jan.
Article in En | MEDLINE | ID: mdl-27802877
We here show adequate species identification for bacterial isolates of the genus Nocardia spp. through VITEK mass spectrometry. Application of a specific sample preparation method in combination with a robust matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) database leads to 94% accurate identification to the species level on a set of 164 isolates. The possibility to identify Nocardia spp. using MALDI-TOF MS will be available in the next release of VITEK MS update (IVD Version 3.0).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteriological Techniques / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Nocardia / Nocardia Infections Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: Diagn Microbiol Infect Dis Year: 2017 Document type: Article Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteriological Techniques / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Nocardia / Nocardia Infections Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: Diagn Microbiol Infect Dis Year: 2017 Document type: Article Country of publication: