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The Analysis of CD83 Expression on Human Immune Cells Identifies a Unique CD83+-Activated T Cell Population.
Ju, Xinsheng; Silveira, Pablo A; Hsu, Wei-Hsun; Elgundi, Zehra; Alingcastre, Renz; Verma, Nirupama D; Fromm, Phillip D; Hsu, Jennifer L; Bryant, Christian; Li, Ziduo; Kupresanin, Fiona; Lo, Tsun-Ho; Clarke, Candice; Lee, Kenneth; McGuire, Helen; Fazekas de St Groth, Barbara; Larsen, Stephen R; Gibson, John; Bradstock, Kenneth F; Clark, Georgina J; Hart, Derek N J.
Affiliation
  • Ju X; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Silveira PA; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Hsu WH; Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
  • Elgundi Z; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Alingcastre R; Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
  • Verma ND; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Fromm PD; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Hsu JL; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Bryant C; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Li Z; Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
  • Kupresanin F; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Lo TH; Institute of Haematology, Royal Prince Alfred Hospital, University of Sydney, Sydney, New South Wales 2050, Australia.
  • Clarke C; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Lee K; Institute of Haematology, Royal Prince Alfred Hospital, University of Sydney, Sydney, New South Wales 2050, Australia.
  • McGuire H; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Fazekas de St Groth B; Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
  • Larsen SR; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Gibson J; ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia.
  • Bradstock KF; Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
  • Clark GJ; Anatomical Pathology Department, Concord Repatriation General Hospital, Sydney, New South Wales 2139, Australia; and.
  • Hart DN; Sydney Medical School, University of Sydney, Sydney, New South Wales 2006, Australia.
J Immunol ; 197(12): 4613-4625, 2016 12 15.
Article in En | MEDLINE | ID: mdl-27837105
CD83 is a member of the Ig gene superfamily, first identified in activated lymphocytes. Since then, CD83 has become an important marker for defining activated human dendritic cells (DC). Several potential CD83 mRNA isoforms have been described, including a soluble form detected in human serum, which may have an immunosuppressive function. To further understand the biology of CD83, we examined its expression in different human immune cell types before and after activation using a panel of mouse and human anti-human CD83 mAb. The mouse anti-human CD83 mAbs, HB15a and HB15e, and the human anti-human CD83 mAb, 3C12C, were selected to examine cytoplasmic and surface CD83 expression, based on their different binding characteristics. Glycosylation of CD83, the CD83 mRNA isoforms, and soluble CD83 released differed among blood DC, monocytes, and monocyte-derived DC, and other immune cell types. A small T cell population expressing surface CD83 was identified upon T cell stimulation and during allogeneic MLR. This subpopulation appeared specifically during viral Ag challenge. We did not observe human CD83 on unstimulated human natural regulatory T cells (Treg), in contrast to reports describing expression of CD83 on mouse Treg. CD83 expression was increased on CD4+, CD8+ T, and Treg cells in association with clinical acute graft-versus-host disease in allogeneic hematopoietic cell transplant recipients. The differential expression and function of CD83 on human immune cells reveal potential new roles for this molecule as a target of therapeutic manipulation in transplantation, inflammation, and autoimmune diseases.
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Collection: 01-internacional Database: MEDLINE Main subject: Dendritic Cells / Immunoglobulins / Membrane Glycoproteins / Monocytes / Antigens, CD / T-Lymphocyte Subsets / T-Lymphocytes, Regulatory / Hematopoietic Stem Cell Transplantation / Graft vs Host Disease Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: J Immunol Year: 2016 Document type: Article Affiliation country: Country of publication:
Search on Google
Add to My VHL
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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Dendritic Cells / Immunoglobulins / Membrane Glycoproteins / Monocytes / Antigens, CD / T-Lymphocyte Subsets / T-Lymphocytes, Regulatory / Hematopoietic Stem Cell Transplantation / Graft vs Host Disease Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: J Immunol Year: 2016 Document type: Article Affiliation country: Country of publication: