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Characterization of the Xanthomonas translucens Complex Using Draft Genomes, Comparative Genomics, Phylogenetic Analysis, and Diagnostic LAMP Assays.
Langlois, Paul A; Snelling, Jacob; Hamilton, John P; Bragard, Claude; Koebnik, Ralf; Verdier, Valérie; Triplett, Lindsay R; Blom, Jochen; Tisserat, Ned A; Leach, Jan E.
Affiliation
  • Langlois PA; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
  • Snelling J; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
  • Hamilton JP; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
  • Bragard C; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
  • Koebnik R; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
  • Verdier V; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
  • Triplett LR; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
  • Blom J; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
  • Tisserat NA; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
  • Leach JE; First, second, sixth, seventh, ninth, and tenth authors: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins 80523-1177; second author: Department of Horticulture, Oregon State University, Corvallis 97331; third author: Department of Plant Biology, Mic
Phytopathology ; 107(5): 519-527, 2017 05.
Article in En | MEDLINE | ID: mdl-28112596
Prevalence of Xanthomonas translucens, which causes cereal leaf streak (CLS) in cereal crops and bacterial wilt in forage and turfgrass species, has increased in many regions in recent years. Because the pathogen is seedborne in economically important cereals, it is a concern for international and interstate germplasm exchange and, thus, reliable and robust protocols for its detection in seed are needed. However, historical confusion surrounding the taxonomy within the species has complicated the development of accurate and reliable diagnostic tools for X. translucens. Therefore, we sequenced genomes of 15 X. translucens strains representing six different pathovars and compared them with additional publicly available X. translucens genome sequences to obtain a genome-based phylogeny for robust classification of this species. Our results reveal three main clusters: one consisting of pv. cerealis, one consisting of pvs. undulosa and translucens, and a third consisting of pvs. arrhenatheri, graminis, phlei, and poae. Based on genomic differences, diagnostic loop-mediated isothermal amplification (LAMP) primers were developed that clearly distinguish strains that cause disease on cereals, such as pvs. undulosa, translucens, hordei, and secalis, from strains that cause disease on noncereal hosts, such as pvs. arrhenatheri, cerealis, graminis, phlei, and poae. Additional LAMP assays were developed that selectively amplify strains belonging to pvs. cerealis and poae, distinguishing them from other pathovars. These primers will be instrumental in diagnostics when implementing quarantine regulations to limit further geographic spread of X. translucens pathovars.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Xanthomonas / Genome, Bacterial / Genomics Type of study: Diagnostic_studies / Guideline / Risk_factors_studies Language: En Journal: Phytopathology Journal subject: BOTANICA Year: 2017 Document type: Article Country of publication:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Diseases / Xanthomonas / Genome, Bacterial / Genomics Type of study: Diagnostic_studies / Guideline / Risk_factors_studies Language: En Journal: Phytopathology Journal subject: BOTANICA Year: 2017 Document type: Article Country of publication: