High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles.
Nat Methods
; 14(3): 283-286, 2017 03.
Article
in En
| MEDLINE
| ID: mdl-28114288
ABSTRACT
Investigation of the structure, assembly and function of protein-nucleic acid macromolecular machines requires multidimensional molecular and structural biology approaches. We describe modifications to an Orbitrap mass spectrometer, enabling high-resolution native MS analysis of 0.8- to 2.3-MDa prokaryotic 30S, 50S and 70S ribosome particles and the 9-MDa Flock House virus. The instrument's improved mass range and sensitivity readily exposes unexpected binding of the ribosome-associated protein SRA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Nodaviridae
/
Escherichia coli
/
Ribosome Subunits, Large, Bacterial
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Ribosome Subunits, Small, Bacterial
/
RNA, Long Noncoding
Language:
En
Journal:
Nat Methods
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2017
Document type:
Article
Affiliation country: