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High-fidelity mass analysis unveils heterogeneity in intact ribosomal particles.
van de Waterbeemd, Michiel; Fort, Kyle L; Boll, Dmitriy; Reinhardt-Szyba, Maria; Routh, Andrew; Makarov, Alexander; Heck, Albert J R.
Affiliation
  • van de Waterbeemd M; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands.
  • Fort KL; Netherlands Proteomics Center, Utrecht, the Netherlands.
  • Boll D; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands.
  • Reinhardt-Szyba M; Netherlands Proteomics Center, Utrecht, the Netherlands.
  • Routh A; Thermo Fisher Scientific, Bremen, Germany.
  • Makarov A; Thermo Fisher Scientific, Bremen, Germany.
  • Heck AJ; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
Nat Methods ; 14(3): 283-286, 2017 03.
Article in En | MEDLINE | ID: mdl-28114288
ABSTRACT
Investigation of the structure, assembly and function of protein-nucleic acid macromolecular machines requires multidimensional molecular and structural biology approaches. We describe modifications to an Orbitrap mass spectrometer, enabling high-resolution native MS analysis of 0.8- to 2.3-MDa prokaryotic 30S, 50S and 70S ribosome particles and the 9-MDa Flock House virus. The instrument's improved mass range and sensitivity readily exposes unexpected binding of the ribosome-associated protein SRA.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mass Spectrometry / Nodaviridae / Escherichia coli / Ribosome Subunits, Large, Bacterial / Ribosome Subunits, Small, Bacterial / RNA, Long Noncoding Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2017 Document type: Article Affiliation country:
Fulltext
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Mass Spectrometry / Nodaviridae / Escherichia coli / Ribosome Subunits, Large, Bacterial / Ribosome Subunits, Small, Bacterial / RNA, Long Noncoding Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2017 Document type: Article Affiliation country: