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Identification of four cornua by ultra-performance liquid chromatography with time-of-flight mass spectrometry coupled with principal component analysis.
Cheng, Xian-Long; Zhang, Qian-Qian; Li, Ming-Hua; Yan, Hua; Yu, Kun-Zi; Zhou, Jian-Li; Li, Shao-Ping; Wei, Feng; Ma, Shuang-Cheng.
Affiliation
  • Cheng XL; Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
  • Zhang QQ; Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
  • Li MH; Department of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.
  • Yan H; Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
  • Yu KZ; Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
  • Zhou JL; Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
  • Li SP; Department of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.
  • Wei F; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
  • Ma SC; Institute for Control of Traditional Chinese Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, China.
J Sep Sci ; 40(8): 1667-1673, 2017 Apr.
Article in En | MEDLINE | ID: mdl-28217946
An ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry method coupled with principal component analysis was developed and applied to the identification of Cornu Antelopis, Cornu Bubali, Cornu Naemorhedi, and Cornu Bovis. The data obtained from the trypsin-digested samples were subjected to principal component analysis to classify these four cornua. Additionally, marker peptides of the cornua were determined by orthogonal partial least-squares discriminant analysis, and fragmentation tandem mass spectra of these marker peptides were evaluated. The results from this study indicate that the proposed method is reliable, and it has been successfully applied to the identification of variants of cornua commonly used in traditional Chinese medicine.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Materia Medica / Horns Type of study: Diagnostic_studies Limits: Animals Language: En Journal: J Sep Sci Year: 2017 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Materia Medica / Horns Type of study: Diagnostic_studies Limits: Animals Language: En Journal: J Sep Sci Year: 2017 Document type: Article Affiliation country: Country of publication: