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Assessment of ALK gene rearrangement in lung cancer using a new rapid automated SureFISH (Dako Omnis) assay.
Takeda, Maiko; Kasai, Takahiko; Shimizu, Shigeki; Kitaichi, Masanori; Kojima, Kensuke; Nagoya, Akihiro; Hayashi, Akio; Utsumi, Tomoki; Yoon, Hyung-Eun; Matsumura, Akihide; Atagi, Shinji.
Affiliation
  • Takeda M; Department of Laboratory Medicine and Pathology, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
  • Kasai T; Department of Laboratory Medicine and Pathology, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
  • Shimizu S; Department of Pathology, Kinki University of Medicine, Osaka-Sayama, Japan.
  • Kitaichi M; Department of Laboratory Medicine and Pathology, National Hospital Organization Minami Wakayama Medical Center, Wakayama, Japan.
  • Kojima K; Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
  • Nagoya A; Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
  • Hayashi A; Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
  • Utsumi T; Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
  • Yoon HE; Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
  • Matsumura A; Department of Surgery, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
  • Atagi S; Department of Thoracic Oncology, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
J Clin Pathol ; 70(8): 712-714, 2017 Aug.
Article in En | MEDLINE | ID: mdl-28264878
ABSTRACT
The ALK fluorescence in situ hybridisation (FISH) method is the examination essential for pathological diagnosis and choice of molecular-targeted therapy in ALK-rearranged lung cancer. Here, for detection of ALK gene rearrangement in patients with lung cancer, we evaluated the rapid FISH technology (ALK SureFISH), a newly developed assay for the automated staining platform Dako Omnis, using 21 formalin-fixed paraffin-embedded (FFPE) samples. All cases could be evaluated with the SureFISH method. SureFISH provided excellent quality signals without any background staining. The SureFISH assay was able to offer a rapid turnaround time (approximately 3.5 hours) and was 100% concordant with prior Vysis FISH results in our laboratory.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Adenocarcinoma / Receptor Protein-Tyrosine Kinases / Carcinoma, Non-Small-Cell Lung / Lung Neoplasms Type of study: Evaluation_studies Limits: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Language: En Journal: J Clin Pathol Year: 2017 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Adenocarcinoma / Receptor Protein-Tyrosine Kinases / Carcinoma, Non-Small-Cell Lung / Lung Neoplasms Type of study: Evaluation_studies Limits: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Language: En Journal: J Clin Pathol Year: 2017 Document type: Article Affiliation country: