Edible leaf extract of Ipomoea aquatica Forssk. (Convolvulaceae) attenuates doxorubicin-induced liver injury via inhibiting oxidative impairment, MAPK activation and intrinsic pathway of apoptosis.
Food Chem Toxicol
; 105: 322-336, 2017 Jul.
Article
in En
| MEDLINE
| ID: mdl-28478100
ABSTRACT
Ipomoea aquatica Forssk. (Convolvulaceae) is an aquatic vegetable traditionally employed against toxic effects of xenobiotics. The present study has been designed to investigate the molecular mechanism underlying the beneficial role of the edible (aqueous) leaf extract of I. aquatica (AEIA) against doxorubicin (Dox)-induced liver injury. AEIA exhibited a dose-dependent (â¼400 µg/ml) increase in cell viability against Dox (1 µM) in isolated rodent hepatocytes. AEIA (400 µg/ml) prevented the Dox-induced increase in ROS, redox imbalance, and activation of mitogen activated protein kinases (MAPK) and intrinsic pathway of apoptosis in hepatocytes. In the in vivo assay, administration of AEIA (100 mg/kg, p.o.) against Dox (3 mg/kg, i.p.) also reduced the oxidative impairment, DNA fragmentation, ATP formation, and up-regulated the mitochondrial co-enzymes Qs in the liver tissues of Wistar rats. Histological assessments were in agreement with the biochemical findings. Substantial quantities of phyto-antioxidants in AEIA may mediate its beneficial function against Dox-induced liver injury.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Extracts
/
Doxorubicin
/
Apoptosis
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Plant Leaves
/
MAP Kinase Signaling System
/
Ipomoea
/
Chemical and Drug Induced Liver Injury
/
Antineoplastic Agents
Type of study:
Etiology_studies
Limits:
Animals
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Humans
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Male
Language:
En
Journal:
Food Chem Toxicol
Year:
2017
Document type:
Article