An In Vitro Model for Fibroblast Photoaging Comparing Single and Repeated UVA Irradiations.
Photochem Photobiol
; 93(6): 1462-1471, 2017 11.
Article
in En
| MEDLINE
| ID: mdl-28599356
ABSTRACT
The current method for efficient evaluation of antiphotoaging compounds is an in vitro skin culture model using a single ultraviolet A (UVA) irradiation of fibroblasts. However, skin photoaging is caused by repeated exposure to UVA radiation. The objective of this study was to develop an appropriate model for in vitro skin photoaging by comparing the different effects of single (5 J cm-2 ) and repeated exposures (5 J cm-2 × 3 times) of fibroblasts to UVA irradiation. Our results demonstrated that a single and repeated exposure to UVA irradiation had different effects on fibroblasts. In the single UVA-irradiated group, collagen lattice contraction and the protein levels of type I procollagen and matrix metalloproteinase-1 (MMP-1) increased, while the levels of fibronectin and alpha-smooth muscle actin (α-SMA) were unchanged, compared to levels in the non-UVA-irradiated group (control). In contrast, repeated UVA exposure significantly induced G0/G1 cell cycle arrest, reduced collagen lattice contraction and type I procollagen and fibronectin expression, and increased MMP-1 expression. There was no difference in α-SMA expression when comparing repeatedly irradiated and non-UVA-irradiated fibroblasts. Our findings clearly indicate that repeated UVA irradiation of cells induces malfunctions found in photoaged skin and is an appropriate in vitro skin model of photoaging.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Ultraviolet Rays
/
Skin Aging
/
Fibroblasts
/
Models, Biological
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Photochem Photobiol
Year:
2017
Document type:
Article
Affiliation country:
Country of publication:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA