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Transcriptomic analysis of purified human cortical microglia reveals age-associated changes.
Galatro, Thais F; Holtman, Inge R; Lerario, Antonio M; Vainchtein, Ilia D; Brouwer, Nieske; Sola, Paula R; Veras, Mariana M; Pereira, Tulio F; Leite, Renata E P; Möller, Thomas; Wes, Paul D; Sogayar, Mari C; Laman, Jon D; den Dunnen, Wilfred; Pasqualucci, Carlos A; Oba-Shinjo, Sueli M; Boddeke, Erik W G M; Marie, Suely K N; Eggen, Bart J L.
Affiliation
  • Galatro TF; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
  • Holtman IR; Department of Neurology, Laboratory of Molecular and Cellular Biology, School of Medicine, University of São Paulo, São Paulo, Brazil.
  • Lerario AM; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
  • Vainchtein ID; Department of Internal Medicine, Division of Metabolism, Endocrinology, and Diabetes, University of Michigan, Ann Arbor, Michigan, USA.
  • Brouwer N; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
  • Sola PR; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
  • Veras MM; Department of Neurology, Laboratory of Molecular and Cellular Biology, School of Medicine, University of São Paulo, São Paulo, Brazil.
  • Pereira TF; Brazilian Aging Brain Study Group, School of Medicine, University of São Paulo, São Paulo, Brazil.
  • Leite REP; Center for Studies of Cellular and Molecular Therapy (NAP-NETCEM-NUCEL), University of São Paulo, São Paulo, Brazil.
  • Möller T; Chemistry Institute, Department of Biochemistry, University of São Paulo, São Paulo, Brazil.
  • Wes PD; Brazilian Aging Brain Study Group, School of Medicine, University of São Paulo, São Paulo, Brazil.
  • Sogayar MC; Neuroinflammation Disease Biology Unit, Lundbeck Research USA, Paramus, New Jersey, USA.
  • Laman JD; Neuroinflammation Disease Biology Unit, Lundbeck Research USA, Paramus, New Jersey, USA.
  • den Dunnen W; Center for Studies of Cellular and Molecular Therapy (NAP-NETCEM-NUCEL), University of São Paulo, São Paulo, Brazil.
  • Pasqualucci CA; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
  • Oba-Shinjo SM; Department of Pathology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
  • Boddeke EWGM; Brazilian Aging Brain Study Group, School of Medicine, University of São Paulo, São Paulo, Brazil.
  • Marie SKN; Department of Neurology, Laboratory of Molecular and Cellular Biology, School of Medicine, University of São Paulo, São Paulo, Brazil.
  • Eggen BJL; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.
Nat Neurosci ; 20(8): 1162-1171, 2017 Aug.
Article in En | MEDLINE | ID: mdl-28671693
Microglia are essential for CNS homeostasis and innate neuroimmune function, and play important roles in neurodegeneration and brain aging. Here we present gene expression profiles of purified microglia isolated at autopsy from the parietal cortex of 39 human subjects with intact cognition. Overall, genes expressed by human microglia were similar to those in mouse, including established microglial genes CX3CR1, P2RY12 and ITGAM (CD11B). However, a number of immune genes, not identified as part of the mouse microglial signature, were abundantly expressed in human microglia, including TLR, Fcγ and SIGLEC receptors, as well as TAL1 and IFI16, regulators of proliferation and cell cycle. Age-associated changes in human microglia were enriched for genes involved in cell adhesion, axonal guidance, cell surface receptor expression and actin (dis)assembly. Limited overlap was observed in microglial genes regulated during aging between mice and humans, indicating that human and mouse microglia age differently.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain / Aging / Gene Expression / Microglia / CD11b Antigen Type of study: Risk_factors_studies Limits: Humans Language: En Journal: Nat Neurosci Journal subject: NEUROLOGIA Year: 2017 Document type: Article Affiliation country: Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain / Aging / Gene Expression / Microglia / CD11b Antigen Type of study: Risk_factors_studies Limits: Humans Language: En Journal: Nat Neurosci Journal subject: NEUROLOGIA Year: 2017 Document type: Article Affiliation country: Country of publication: