EPR Technology as Sensitive Method for Oxidative Stress Detection in Primary and Secondary Keratinocytes Induced by Two Selected Nanoparticles.
Cell Biochem Biophys
; 75(3-4): 359-367, 2017 Dec.
Article
in En
| MEDLINE
| ID: mdl-28849322
ABSTRACT
Exogenous factors can cause an imbalance in the redox state of biological systems, promoting the development of oxidative stress, especially reactive oxygen species (ROS). To monitor the intensity of ROS production in secondary keratinocytes (HaCaT) by diesel exhaust particles and thermoresponsive nanogels (tNG), electron paramagnetic resonance (EPR) spectroscopy after 1 and 24 h of incubation, respectively, was applied. Their cytotoxicity was analyzed by a cell viability assay (XTT). For tNG an increase in the cell viability and ROS production of 10% was visible after 24 h, whereas 1 h showed no effect. A ten times lower concentration of diesel exhaust particles exhibited no significant toxic effects on HaCaT cells for both incubation times, thus normal adult human keratinocytes (NHK) were additionally analyzed by XTT and EPR spectroscopy. Here, after 24 h a slight increase of 18% in metabolic activity was observed. However, this effect could not be explained by the ROS formation. A slight increase in the ROS production was only visible after 1 h of incubation time for HaCaT (9%) and NHK (14%).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Reactive Oxygen Species
/
Electron Spin Resonance Spectroscopy
/
Oxidative Stress
/
Nanoparticles
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Cell Biochem Biophys
Journal subject:
BIOFISICA
/
BIOQUIMICA
Year:
2017
Document type:
Article
Affiliation country: