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Combined effect of retinoic acid and calcium on the in vitro differentiation of human adipose-derived stem cells to adipocytes.
Goudarzi, Farjam; Sarveazad, Arash; Mahmoudi, Maryam; Mohammadalipour, Adel; Chahardoli, Reza; Malekshah, Obeid M; Karimi Gorgani, Shiva; Saboor-Yaraghi, Ali Akbar.
Affiliation
  • Goudarzi F; a Department of Biochemistry, School of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.
  • Sarveazad A; b Colorectal Research Center , Iran University of Medical Sciences , Tehran , Iran.
  • Mahmoudi M; c Department of Cellular Molecular Nutrition, School of Nutritional Sciences and Dietetics , Tehran University of Medical Sciences , Tehran , Iran.
  • Mohammadalipour A; a Department of Biochemistry, School of Medicine , Hamadan University of Medical Sciences , Hamadan , Iran.
  • Chahardoli R; c Department of Cellular Molecular Nutrition, School of Nutritional Sciences and Dietetics , Tehran University of Medical Sciences , Tehran , Iran.
  • Malekshah OM; d Department of Pharmaceutics, Ernest Mario School of Pharmacy , Rutgers, The State University of New Jersey , Piscataway , NJ , USA.
  • Karimi Gorgani S; e Health Information Management Department , Paramedical School, Kermanshah University of Medical Sciences , Kermanshah , Iran.
  • Saboor-Yaraghi AA; f Department of Immunology, School of Public Health , Tehran University of Medical sciences , Tehran , Iran.
Arch Physiol Biochem ; 124(2): 109-118, 2018 May.
Article in En | MEDLINE | ID: mdl-28850272
CONTEXT: It has been shown that adipogenesis can be modulated by factors such as all-trans retinoic acid (ATRA) and calcium. OBJECTIVE: To determine, the combined effect of ATRA and calcium on the differentiation of human adipose-derived stem cells (hADSCs). METHODS: Mesenchymal stem cells (MSCs) were differentiated into the adipocytes by 0.5 and 1 µM of ATRA and 5 and 10 mM calcium separately or in combination. After MTS assay the differentiation of MSCs to adipocyte was evaluated, Oil Red O staining, GLUT4 concentration and gene expression of PPARG2, adiponectin, and GLUT4 were measured by Real-Time PCR. RESULTS: Except 10 mM calcium treated group, other groups and more significantly combination treatments could reduce all adipocyte markers compared to the control. CONCLUSION: These results suggest that ATRA and calcium together have significant inhibitory effect on adipogenesis that can be helpful for finding new mechanisms to prevent or control the adipogenesis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tretinoin / Down-Regulation / Calcium Signaling / Subcutaneous Fat, Abdominal / Adipogenesis / Adult Stem Cells / Obesity Limits: Adult / Female / Humans Language: En Journal: Arch Physiol Biochem Journal subject: BIOQUIMICA / FISIOLOGIA Year: 2018 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tretinoin / Down-Regulation / Calcium Signaling / Subcutaneous Fat, Abdominal / Adipogenesis / Adult Stem Cells / Obesity Limits: Adult / Female / Humans Language: En Journal: Arch Physiol Biochem Journal subject: BIOQUIMICA / FISIOLOGIA Year: 2018 Document type: Article Affiliation country: Country of publication: