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Neither linoleic acid nor arachidonic acid promote white adipose tissue inflammation in Fads2-/- mice fed low fat diets.
Suitor, Katherine; Payne, George W; Sarr, Ousseynou; Abdelmagid, Salma; Nakamura, Manabu T; Ma, David Wl; Mutch, David M.
Affiliation
  • Suitor K; Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Rd. E., Guelph, ON, Canada N1G2W1.
  • Payne GW; Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Rd. E., Guelph, ON, Canada N1G2W1.
  • Sarr O; Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Rd. E., Guelph, ON, Canada N1G2W1.
  • Abdelmagid S; Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Rd. E., Guelph, ON, Canada N1G2W1.
  • Nakamura MT; Division of Nutritional Sciences, University of Illinois, 905 South Goodwin Avenue, Urbana, IL 61801, USA.
  • Ma DW; Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Rd. E., Guelph, ON, Canada N1G2W1.
  • Mutch DM; Department of Human Health and Nutritional Sciences, University of Guelph, 50 Stone Rd. E., Guelph, ON, Canada N1G2W1. Electronic address: dmutch@uoguelph.ca.
Article in En | MEDLINE | ID: mdl-29031400
Dietary n-6 polyunsaturated fatty acids (PUFA) are widely perceived to promote inflammation and contribute to the development of chronic diseases. This dogma has been recently questioned due to evidence that n-6 PUFA, specifically linoleic acid (LA, 18:2n-6) and arachidonic acid (AA, 20:4n-6), do not appear to activate inflammatory signalling pathways when consumed in moderate amounts. However, delineating the independent roles of different dietary n-6 PUFA in vivo is challenging because LA is continuously converted into AA in a pathway regulated by the fatty acid desaturase 2 (Fads2) gene. The objective of this study was to investigate the independent roles of LA and AA on white adipose tissue (WAT) inflammatory signalling pathways using Fads2-/- mice. We hypothesized that dietary LA would not induce WAT inflammation, unless it was endogenously converted into AA. Male C57BL/6 wild-type (WT) and Fads2-/- mice were fed low-fat isocaloric diets containing either 7% corn oil w/w (CD, containing ~42% LA) or 7% ARASCO oil w/w (AD, containing ~27% AA) for 9 weeks. WAT inflammatory gene expression, protein levels, as well as phospholipid (PL) and triacylglycerol (TAG) fatty acid composition, were analyzed by RT-qPCR, western blots, and gas chromatography, respectively. Fads2-/- mice fed CD had high LA, but little-to-no GLA (18:3n-6), DGLA (20:3n-6), and AA in PLs and TAGs compared to their WT counterparts. In comparison, Fads2-/- and WT mice fed AD showed minimal differences in n-6 PUFA content in serum and WAT, despite having significantly more AA than CD-fed mice. No differences in gene expression for common inflammatory adipokines (e.g. Mcp-1, Ccl5, Tnfα) or key regulators of eicosanoid production (e.g. Cox-2, Alox-12, Alox-15) were detected in WAT between any of the diet and genotype groups. Furthermore, no differences in MCP-1, and total or phosphorylated STAT3 and p38 inflammatory proteins, were observed. Collectively, these results demonstrate that neither LA nor AA promote WAT inflammation when consumed as part of a low-fat diet. Therefore, the existing dogma surrounding n-6 PUFA and inflammation needs to be reconsidered.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arachidonic Acid / Linoleic Acid / Fatty Acid Desaturases / Inflammation Limits: Animals Language: En Journal: Prostaglandins Leukot Essent Fatty Acids Journal subject: ENDOCRINOLOGIA Year: 2017 Document type: Article Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arachidonic Acid / Linoleic Acid / Fatty Acid Desaturases / Inflammation Limits: Animals Language: En Journal: Prostaglandins Leukot Essent Fatty Acids Journal subject: ENDOCRINOLOGIA Year: 2017 Document type: Article Country of publication: