Architecture of eukaryotic mRNA 3'-end processing machinery.
Science
; 358(6366): 1056-1059, 2017 11 24.
Article
in En
| MEDLINE
| ID: mdl-29074584
ABSTRACT
Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by the ~1-megadalton multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination, but it is unclear how its various enzymes are coordinated and assembled. Here, we show that the nuclease, polymerase, and phosphatase activities of yeast CPF are organized into three modules. Using electron cryomicroscopy, we determined a 3.5-angstrom-resolution structure of the ~200-kilodalton polymerase module. This revealed four ß propellers, in an assembly markedly similar to those of other protein complexes that bind nucleic acid. Combined with in vitro reconstitution experiments, our data show that the polymerase module brings together factors required for specific and efficient polyadenylation, to help coordinate mRNA 3'-end processing.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
RNA Polymerase II
/
RNA, Messenger
/
Saccharomyces cerevisiae Proteins
/
RNA 3' End Processing
/
MRNA Cleavage and Polyadenylation Factors
Language:
En
Journal:
Science
Year:
2017
Document type:
Article
Affiliation country: