Your browser doesn't support javascript.
loading
Novel Microbial Diversity and Functional Potential in the Marine Mammal Oral Microbiome.
Dudek, Natasha K; Sun, Christine L; Burstein, David; Kantor, Rose S; Aliaga Goltsman, Daniela S; Bik, Elisabeth M; Thomas, Brian C; Banfield, Jillian F; Relman, David A.
Affiliation
  • Dudek NK; Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
  • Sun CL; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Burstein D; Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Kantor RS; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Aliaga Goltsman DS; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Bik EM; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Thomas BC; Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Banfield JF; Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720, USA; Earth and Environmental Science, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  • Relman DA; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA. Electronic address: relman@sta
Curr Biol ; 27(24): 3752-3762.e6, 2017 Dec 18.
Article in En | MEDLINE | ID: mdl-29153320
The vast majority of bacterial diversity lies within phylum-level lineages called "candidate phyla," which lack isolated representatives and are poorly understood. These bacteria are surprisingly abundant in the oral cavity of marine mammals. We employed a genome-resolved metagenomic approach to recover and characterize genomes and functional potential from microbes in the oral gingival sulcus of two bottlenose dolphins (Tursiops truncatus). We detected organisms from 24 known bacterial phyla and one archaeal phylum. We also recovered genomes from two deep-branching, previously uncharacterized phylum-level lineages (here named "Candidatus Delphibacteria" and "Candidatus Fertabacteria"). The Delphibacteria lineage is found in both managed and wild dolphins; its metabolic profile suggests a capacity for denitrification and a possible role in dolphin health. We uncovered a rich diversity of predicted Cas9 proteins, including the two longest predicted Cas9 proteins to date. Notably, we identified the first type II CRISPR-Cas systems encoded by members of the Candidate Phyla Radiation. Using their spacer sequences, we subsequently identified and assembled a complete Saccharibacteria phage genome. These findings underscore the immense microbial diversity and functional potential that await discovery in previously unexplored environments.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteria / Genome, Bacterial / Archaea / Genome, Archaeal / Bottle-Nosed Dolphin / Metagenome / Microbiota Type of study: Prognostic_studies Limits: Animals Language: En Journal: Curr Biol Journal subject: BIOLOGIA Year: 2017 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteria / Genome, Bacterial / Archaea / Genome, Archaeal / Bottle-Nosed Dolphin / Metagenome / Microbiota Type of study: Prognostic_studies Limits: Animals Language: En Journal: Curr Biol Journal subject: BIOLOGIA Year: 2017 Document type: Article Affiliation country: Country of publication: