Generation and characterization of a fowl adenovirus 9 dual-site expression vector.
J Biotechnol
; 266: 102-110, 2018 Jan 20.
Article
in En
| MEDLINE
| ID: mdl-29269248
ABSTRACT
Fowl adenoviruses (FAdVs) are widely considered as excellent platforms for vaccine development and gene therapy. We improved on our right-end partial TR-2 deleted or a left-end 2.3â¯kb deleted vectors by developing a single, dual-site delivery vector. We demonstrated that, in addition to ORF11, the right end ORF17 is also dispensable. To further improve the capacity and flexibility of the FAdV-9 based vector system, we generated an infectious recombinant FAdV-9 dual-site expression clone lacking 1.9â¯kb of the left end and replaced with mCherry under the control of a native promoter, and 3.6â¯kb of the right-end replaced with an EGFP expression cassette. Five intermediate FAdmid clones were successfully constructed a) pFAdV-9Δ0-2RED (mCherry replacing the left end 2.2â¯kb ORF0 to 2); b) pFAdV-9RED (mCherry replacing the left end 1.9â¯kb ORF1 to 2); c) pFAdV-9Δ17 (deletion of ORF17 and 393â¯bp downstream untranslated region); d) pFAdV-9GFP (EGFP expression cassette replacing the right end 3.6â¯kb) and e) pFAdV-9Dual (both mCherry in the left end and the EGFP expression cassette in the right end of our vector). Our novel FAdV-9 dual-site vaccine vector, produced infectious virus and expressed either one or both mCherry and EGFP.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Expression
/
Aviadenovirus
/
Genetic Vectors
Limits:
Animals
Language:
En
Journal:
J Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2018
Document type:
Article
Affiliation country: