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Expression and immunolocalization of metallothioneins MT1, MT2 and MT3 in rat nephron.
Sabolic, Ivan; Skarica, Mario; Ljubojevic, Marija; Breljak, Davorka; Herak-Kramberger, Carol M; Crljen, Vladiana; Ljubesic, Nikola.
Affiliation
  • Sabolic I; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Croatia. Electronic address: sabolic@imi.hr.
  • Skarica M; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Croatia.
  • Ljubojevic M; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Croatia.
  • Breljak D; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Croatia.
  • Herak-Kramberger CM; Molecular Toxicology Unit, Institute for Medical Research and Occupational Health, Croatia.
  • Crljen V; Croatian Institute for Brain Research & Department of Physiology, School of Medicine, University of Zagreb, Croatia.
  • Ljubesic N; Croatian Academy of Sciences and Arts (HAZU), Zagreb, Croatia.
J Trace Elem Med Biol ; 46: 62-75, 2018 Mar.
Article in En | MEDLINE | ID: mdl-29413112
Rodent kidneys exhibit three isoforms of metallothioneins (MTs), MT1, MT2 and MT3, with poorly characterized localization along the nephron. Here we studied in adult male Wistar rats the renal expression of MTs mRNA by end-point RT-PCR and MT proteins by immunochemical methods The expression pattern of MT1 mRNA was cortex (CO)>outer stripe (OS)=inner stripe (IS)=inner medulla (IM), of MT2 mRNA was IM>CO>IS=OS, and of MT3 mRNA was IM>CO=OS=IM. MT1/2-antibody stained with heterogeneous intensity the cell cytoplasm and nuclei in proximal tubule (PT) and thin ascending limb, whereas MT3-antibody stained weakly the cell cytoplasm in various cortical tubules and strongly the nuclei in all nephron segments. However, the isolated nuclei exhibited an absence of MT1/2 and presence of MT3 protein. In MT1/2-positive PT cells, the intracellular staining appeared diffuse or bipolar, but the isolated brush-border, basolateral and endosomal membranes were devoid of MT1/2 proteins. In the lumen of some PT profiles, the heterogeneously sized MT1/2-rich vesicles were observed, with the limiting membrane positive for NHE3, but negative for V-ATPase, CAIV, and megalin, whereas their interior was positive for CAII and negative for cytoskeleton. They seem to be pinched off from the luminal membrane of MT1/2-rich cells, as also indicated by transmission electron microscopy. We conclude that in male rats, MTs are heterogeneously abundant in the cell cytoplasm and/or nuclei along the nephron. The MT1/2-rich vesicles in the tubule lumen may represent a source of urine MT and membranous material, whereas MT3 in nuclei may handle zink and locally-produced reactive oxygen species.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Metallothionein / Nephrons / Nerve Tissue Proteins Limits: Animals Language: En Journal: J Trace Elem Med Biol Journal subject: METABOLISMO / SAUDE AMBIENTAL Year: 2018 Document type: Article Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Metallothionein / Nephrons / Nerve Tissue Proteins Limits: Animals Language: En Journal: J Trace Elem Med Biol Journal subject: METABOLISMO / SAUDE AMBIENTAL Year: 2018 Document type: Article Country of publication: