Establishment of an evaluation method to detect drug distribution in hair follicles.
Int J Pharm
; 542(1-2): 27-35, 2018 May 05.
Article
in En
| MEDLINE
| ID: mdl-29471145
ABSTRACT
Development of an appropriate method to evaluate drug disposition or targeting ability in hair follicles (HFs) is urgently needed in order to develop useful pharmaceutical products with pharmacological effects in HFs. In the present study, a cyanoacrylate biopsy (CB) method was used to measure drug disposition in HFs using a model hydrophilic drug, caffeine (CAF), and a lipophilic drug, 4-butylresorcinol (BR), in excised porcine skin. As a result, the height of HF replicas and the recovery ratio decreased with an increase in the application times of the CB method. HF replicas with a length of approximately 175⯵m were obtained using a single application of the CB method. Drug distribution in the HF was detected even 5â¯min after topical application regardless of the lipophilicity of the drugs, although no drug disposition was observed in the deeper layers of the stratum corneum at the same time (5â¯min). Furthermore, significantly higher amounts of BR were observed in the stratum corneum and HF, compared with those of CAF. These results suggested that the CB method could be useful to evaluate the safety and efficacy as well as the disposition of topically applied chemicals, especially for HF-targeting drugs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Resorcinols
/
Caffeine
/
Hair Follicle
Limits:
Animals
Language:
En
Journal:
Int J Pharm
Year:
2018
Document type:
Article
Affiliation country: