Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol
; 92(7): 2385-2399, 2018 07.
Article
in En
| MEDLINE
| ID: mdl-29855658
ABSTRACT
The genetically modified maize event MON810 expresses a Bacillus thuringiensis-derived gene, which encodes the insecticidal protein Cry1Ab to control some lepidopteran insect pests such as the European corn borer. It has been claimed that the immune system may be affected following the oral/intragastric administration of the MON810 maize in various different animal species. In the frame of the EU-funded project GRACE, two 90-day feeding trials, the so-called studies D and E, were performed to analyze the humoral and cellular immune responses of male and female Wistar Han RCC rats fed the MON810 maize. A MON810 maize variety of Monsanto was used in the study D and a MON810 maize variety of Pioneer Hi-Bred was used in the study E. The total as well as the maize protein- and Cry1Ab-serum-specific IgG, IgM, IgA and IgE levels, the proliferative activity of the lymphocytes, the phagocytic activity of the granulocytes and monocytes, the respiratory burst of the phagocytes, a phenotypic analysis of spleen, thymus and lymph node cells as well as the in vitro production of cytokines by spleen cells were analyzed. No specific Cry1Ab immune response was observed in MON810 rats, and anti-maize protein antibody responses were similar in MON810 and control rats. Single parameters were sporadically altered in rats fed the MON810 maize when compared to control rats, but these alterations are considered to be of no immunotoxicological significance.
Key words
Acquired immunity analysis; Anti-Cry1Ab antibodies; Anti-maize protein antibodies; Cellular immune response; Cry1Ab; Food allergenicity; GRACE; Genetically modified maize MON810; Humoral immune response; Immune cell phenotyping; Native immunity analysis; OECD Test Guideline no. 408repeated dose 90-day oral toxicity study in rodents (1998)
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plants, Genetically Modified
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Zea mays
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Food, Genetically Modified
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Immunity, Humoral
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Immunity, Cellular
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Animal Feed
Limits:
Animals
Language:
En
Journal:
Arch Toxicol
Year:
2018
Document type:
Article
Affiliation country: