Candida albicans Cdc15 is essential for mitotic exit and cytokinesis.
Sci Rep
; 8(1): 8899, 2018 06 11.
Article
in En
| MEDLINE
| ID: mdl-29891974
Candida albicans displays a variety of morphological forms, and the ability to switch forms must be linked with cell cycle control. In budding yeast the Mitotic Exit Network (MEN) acts to drive mitotic exit and signal for cytokinesis and cell separation. However, previous reports on the MEN in C. albicans have raised questions on its role in this organism, with the components analysed to date demonstrating differing levels of importance in the processes of mitotic exit, cytokinesis and cell separation. This work focuses on the role of the Cdc15 kinase in C. albicans and demonstrates that, similar to Saccharomyces cerevisiae, it plays an essential role in signalling for mitotic exit and cytokinesis. Cells depleted of Cdc15 developed into elongated filaments, a common response to cell cycle arrest in C. albicans. These filaments emerged exclusively from large budded cells, contained two nuclear bodies and exhibited a hyper-extended spindle, all characteristic of these cells failing to exit mitosis. Furthermore these filaments displayed a clear cytokinesis defect, and CDC15 over-expression led to aberrant cell separation following hyphal morphogenesis. Together, these results are consistent with Cdc15 playing an essential role in signalling for mitotic exit, cytokinesis and cell separation in C. albicans.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Candida albicans
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Cell Cycle Proteins
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GTP-Binding Proteins
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Cytokinesis
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Mitosis
Language:
En
Journal:
Sci Rep
Year:
2018
Document type:
Article
Country of publication: