Tracking disease resistance deployment in potato breeding by enrichment sequencing.
Plant Biotechnol J
; 17(2): 540-549, 2019 02.
Article
in En
| MEDLINE
| ID: mdl-30107090
ABSTRACT
Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the high-confidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans) gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Diseases
/
Plant Proteins
/
Polymorphism, Genetic
/
Solanum tuberosum
/
Phytophthora infestans
/
Disease Resistance
Language:
En
Journal:
Plant Biotechnol J
Journal subject:
BIOTECNOLOGIA
/
BOTANICA
Year:
2019
Document type:
Article
Affiliation country: