Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry.
Anal Bioanal Chem
; 411(3): 559-564, 2019 Jan.
Article
in En
| MEDLINE
| ID: mdl-30109381
ABSTRACT
In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Immunoassay
/
Metal Nanoparticles
/
Gold
/
Lasers
/
Antibodies
Language:
En
Journal:
Anal Bioanal Chem
Year:
2019
Document type:
Article
Affiliation country: