A two-hybrid antibody micropattern assay reveals specific in cis interactions of MHC I heavy chains at the cell surface.
Elife
; 72018 09 05.
Article
in En
| MEDLINE
| ID: mdl-30180933
ABSTRACT
We demonstrate a two-hybrid assay based on antibody micropatterns to study protein-protein interactions at the cell surface of major histocompatibility complex class I (MHC I) proteins. Anti-tag and conformation-specific antibodies are used for individual capture of specific forms of MHC I proteins that allow for location- and conformation-specific analysis by fluorescence microscopy. The assay is used to study the in cis interactions of MHC I proteins at the cell surface under controlled conditions and to define the involved protein conformations. Our results show that homotypic in cis interactions occur exclusively between MHC I free heavy chains, and we identify the dissociation of the light chain from the MHC I protein complex as a condition for MHC I in cis interactions. The functional role of these MHC I protein-protein interactions at the cell surface needs further investigation. We propose future technical developments of our two-hybrid assay for further analysis of MHC I protein-protein interactions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Histocompatibility Antigens Class I
/
Cell Membrane
/
Two-Hybrid System Techniques
/
Antibodies
Limits:
Animals
Language:
En
Journal:
Elife
Year:
2018
Document type:
Article
Affiliation country: