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Evaluation of a conjugate vaccine platform against enterotoxigenic Escherichia coli (ETEC), Campylobacter jejuni and Shigella.
Laird, Renee M; Ma, Zuchao; Dorabawila, Nelum; Pequegnat, Brittany; Omari, Eman; Liu, Yang; Maue, Alexander C; Poole, Steven T; Maciel, Milton; Satish, Kavyashree; Gariepy, Christina L; Schumack, Nina M; McVeigh, Annette L; Poly, Frédéric; Ewing, Cheryl P; Prouty, Michael G; Monteiro, Mario A; Savarino, Stephen J; Guerry, Patricia.
Affiliation
  • Laird RM; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA. Electronic address: renee.m.laird.ctr@mail.mil.
  • Ma Z; Department of Chemistry, University of Guelph, 50 Stone Road E, Guelph, Ontario N1G 2W1, Canada.
  • Dorabawila N; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Pequegnat B; Department of Chemistry, University of Guelph, 50 Stone Road E, Guelph, Ontario N1G 2W1, Canada.
  • Omari E; Department of Chemistry, University of Guelph, 50 Stone Road E, Guelph, Ontario N1G 2W1, Canada.
  • Liu Y; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Maue AC; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Poole ST; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Maciel M; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Satish K; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Gariepy CL; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Schumack NM; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • McVeigh AL; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Poly F; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Ewing CP; Henry M. Jackson Foundation for Military Medicine, 6720A Rockledge Drive, Bethesda, MD 20817, USA; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Prouty MG; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Monteiro MA; Department of Chemistry, University of Guelph, 50 Stone Road E, Guelph, Ontario N1G 2W1, Canada.
  • Savarino SJ; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
  • Guerry P; Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA. Electronic address: patriciaguerry@yahoo.com.
Vaccine ; 36(45): 6695-6702, 2018 10 29.
Article in En | MEDLINE | ID: mdl-30269917
ABSTRACT
Enterotoxigenic Escherichia coli (ETEC), Campylobacter jejuni (CJ), and Shigella sp. are major causes of bacterial diarrhea worldwide, but there are no licensed vaccines against any of these pathogens. Most current approaches to ETEC vaccines are based on recombinant proteins that are involved in virulence, particularly adhesins. In contrast, approaches to Shigella and CJ vaccines have included conjugate vaccines in which Shigella lipopolysaccharides (LPS) or CJ capsule polysaccharides are chemically conjugated to proteins. We have explored the feasibility of developing a multi-pathogen vaccine by using ETEC proteins as conjugating partners for CJ and Shigella polysaccharides. We synthesized three vaccines in which two CJ polysaccharides were conjugated to two recombinant ETEC adhesins based on CFA/I (CfaEB) and CS6 (CssBA), and LPS from Shigella flexneri was also conjugated to CfaEB. The vaccines were immunogenic in mice as monovalent, bivalent and trivalent formulations. Importantly, functional antibodies capable of inducing hemaglutination inhibition (HAI) of a CFA/I expressing ETEC strain were induced in all vaccines containing CfaEB. These data suggest that conjugate vaccines could be a platform for a multi-pathogen, multi-serotype vaccine against the three major causes of diarrheal disease worldwide.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Shigella / Campylobacter jejuni / Vaccines, Conjugate / Enterotoxigenic Escherichia coli Limits: Animals Language: En Journal: Vaccine Year: 2018 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Shigella / Campylobacter jejuni / Vaccines, Conjugate / Enterotoxigenic Escherichia coli Limits: Animals Language: En Journal: Vaccine Year: 2018 Document type: Article