miRNA-448 inhibits cell growth by targeting BCL-2 in hepatocellular carcinoma.
Dig Liver Dis
; 51(5): 703-711, 2019 05.
Article
in En
| MEDLINE
| ID: mdl-30316787
ABSTRACT
BACKGROUND:
Increasing evidence indicates that aberrant micro (mi)RNA-448 expression plays a critical role in the progression of several human cancers. However, the function of miRNA-448 in hepatocellular carcinoma (HCC) has not been fully investigated.METHODS:
miRNA-448 expression levels in HCC tissues, adjacent non-cancerous tissues (ANTs), and HCC cell lines were examined by quantitative real-time polymerase chain reaction (qRT-PCR). HCC cells were treated with a miRNA-448 mimic or inhibitor, followed by cell viability measurements with the CCK-8 assay. Venn diagram analysis predicted, and dual luciferase reporter assays verified, the target gene of miRNA-448. Expression of the target gene was detected by qRT-PCR and immunohistochemistry. Growth of miRNA-448- or target gene-expressing HCC xenograft tumors in nude mice was measured.RESULTS:
miRNA-448 was expressed at a lower level in HCC tissues than ANTs, and correlated with a larger tumor size, incomplete tumor encapsulation, and advanced Barcelona Clinic Liver Cancer stage. miRNA-448 inhibited HCC cell growth. The downstream target of miRNA-448 was BCL-2, which was highly expressed in HCC tissues and its mRNA level was negatively correlated with miRNA-448 expression. In vivo, BCL-2 attenuated the tumor inhibiting effect of miRNA-448.CONCLUSION:
miRNA-448 functions as a tumor suppressor by targeting BCL-2 in HCC.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carcinoma, Hepatocellular
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Proto-Oncogene Proteins c-bcl-2
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MicroRNAs
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Cell Proliferation
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Liver Neoplasms
Limits:
Animals
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Female
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Humans
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Male
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Middle aged
Language:
En
Journal:
Dig Liver Dis
Journal subject:
GASTROENTEROLOGIA
Year:
2019
Document type:
Article
Affiliation country: