Analysis of butterfly reproductive proteins using capillary electrophoresis and mass spectrometry.
Anal Biochem
; 566: 23-26, 2019 02 01.
Article
in En
| MEDLINE
| ID: mdl-30423321
ABSTRACT
A method for analysis of proteins from spermatophores transferred from male to female Pieris napi butterflies during mating has been developed. The proteins were solubilized from the dissected spermatophores using different solubilization agents (water, methanol, acetonitrile and hexafluoroisopropanol). Capillary electrophoresis (CE) analysis was performed using an acidic background electrolyte containing a fluorosurfactant to avoid protein-wall adsorption, and to increase separation performance. The samples were also analyzed with matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), in a lower m/z range (1000-6000) and a higher m/z range (6000-12000). Solubilization with different solvents and the use of alternative matrices gave partly complementary profiles.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatogonia
/
Butterflies
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Electrophoresis, Capillary
/
Insect Proteins
Limits:
Animals
Language:
En
Journal:
Anal Biochem
Year:
2019
Document type:
Article
Affiliation country: