Frequency-dependent lipid raft uptake at rat diaphragm muscle axon terminals.
Muscle Nerve
; 59(5): 611-618, 2019 05.
Article
in En
| MEDLINE
| ID: mdl-30677149
ABSTRACT
INTRODUCTION:
In motor neurons, cholera toxin B (CTB) binds to the cell-surface ganglioside GM1 and is internalized and transported via structurally unique components of plasma membranes (lipid rafts).METHODS:
Lipid raft uptake by axon terminals adjoining type-identified rat diaphragm muscle fibers was investigated using CTB and confocal imaging.RESULTS:
Lipid raft uptake increased significantly at higher frequency stimulation (80 Hz), compared with lower frequency (20 Hz) and unstimulated (0 Hz) conditions. The fraction of axon terminal occupied by CTB was â¼45% at 0- or 20-Hz stimulation, and increased to â¼65% at 80 Hz. Total CTB fluorescence intensity also increased (â¼20%) after 80-Hz stimulation compared with 0 Hz.DISCUSSION:
Evidence of increased lipid raft uptake at high stimulation frequencies supports an important role for lipid raft signaling at rat diaphragm muscle axon terminals, primarily for motor units physiologically activated at the higher frequencies. Muscle Nerve 59611-611, 2019.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phrenic Nerve
/
Diaphragm
/
Cholera Toxin
/
Presynaptic Terminals
/
Membrane Microdomains
/
Neuromuscular Junction
Limits:
Animals
Language:
En
Journal:
Muscle Nerve
Year:
2019
Document type:
Article
Affiliation country: