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Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosis.
Pigna, Eva; Simonazzi, Elena; Sanna, Krizia; Bernadzki, Krzysztof Marian; Proszynski, Tomek; Heil, Constantin; Palacios, Daniela; Adamo, Sergio; Moresi, Viviana.
Affiliation
  • Pigna E; DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, Italy.
  • Simonazzi E; DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, Italy.
  • Sanna K; DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, Italy.
  • Bernadzki KM; Laboratory of Synaptogenesis, Nencki Institute of Experimental Biology Polish Academy of Sciences, Pasteur 3, 02-093 Warsaw, Poland.
  • Proszynski T; Laboratory of Synaptogenesis, Nencki Institute of Experimental Biology Polish Academy of Sciences, Pasteur 3, 02-093 Warsaw, Poland.
  • Heil C; IRCCS Fondazione Santa Lucia, Via del Fosso di Fiorano 64, 00143 Rome, Italy.
  • Palacios D; IRCCS Fondazione Santa Lucia, Via del Fosso di Fiorano 64, 00143 Rome, Italy.
  • Adamo S; DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, Italy.
  • Moresi V; DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, Italy. Electronic address: viviana.moresi@uniroma1.it.
EBioMedicine ; 40: 717-732, 2019 Feb.
Article in En | MEDLINE | ID: mdl-30713114
ABSTRACT

BACKGROUND:

Histone deacetylase 4 (HDAC4) has been proposed as a target for Amyotrophic Lateral Sclerosis (ALS) because it mediates nerve-skeletal muscle interaction and since its expression in skeletal muscle correlates with the severity of the disease. However, our recent studies on the skeletal muscle response upon long-term denervation highlighted the importance of HDAC4 in maintaining muscle integrity.

METHODS:

To fully identify the yet uncharacterized HDAC4 functions in ALS, we genetically deleted HDAC4 in skeletal muscles of a mouse model of ALS. Body weight, skeletal muscle, innervation and spinal cord were analyzed over time by morphological and molecular analyses. Transcriptome analysis was also performed to delineate the signaling modulated by HDAC4 in skeletal muscle of a mouse model of ALS.

FINDINGS:

HDAC4 deletion in skeletal muscle caused earlier ALS onset, characterized by body weight loss, muscle denervation and atrophy, and compromised muscle performance, although the main catabolic pathways were not activated. Transcriptome analysis identified the gene networks modulated by HDAC4 in ALS, revealing UCP1 as a top regulator that may be implicated in worsening ALS features.

INTERPRETATION:

HDAC4 plays an important role in preserving innervations and skeletal muscle in ALS, likely by modulating the UCP1 gene network. Our study highlights a possible risk in considering HDAC inhibitors for the treatment of ALS. FUND This work was supported by FIRB grant (RBFR12BUMH) from Ministry of Education, Universities and Research, by Fondazione Veronesi, by Sapienza research project 2017 (RM11715C78539BD8) and Polish National Science Center grant (UMO-2016/21/B/NZ3/03638).
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Muscular Atrophy / Muscle, Skeletal / Histone Deacetylases / Amyotrophic Lateral Sclerosis Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Journal: EBioMedicine Year: 2019 Document type: Article Affiliation country:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Muscular Atrophy / Muscle, Skeletal / Histone Deacetylases / Amyotrophic Lateral Sclerosis Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Journal: EBioMedicine Year: 2019 Document type: Article Affiliation country: