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Reduction sensitive hyaluronan-SS-poly(ε-caprolactone) block copolymers as theranostic nanocarriers for tumor diagnosis and treatment.
Yang, Huikang; Wang, Nianhua; Mo, Lei; Wu, Mei; Yang, Ruimeng; Xu, Xiangdong; Huang, Yugang; Lin, Jiantao; Zhang, Li-Ming; Jiang, Xinqing.
Affiliation
  • Yang H; Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510640, China.
  • Wang N; Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.
  • Mo L; Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510640, China.
  • Wu M; Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510640, China.
  • Yang R; Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510640, China.
  • Xu X; Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510640, China.
  • Huang Y; School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, China. Electronic address: hyug@gzhmu.edu.cn.
  • Lin J; Dongguan Scientific Research Center, Guangdong Medical University, Dongguan 523808, China.
  • Zhang LM; School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China. Electronic address: ceszhlm@mail.sysu.edu.cn.
  • Jiang X; Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China; Department of Radiology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong 510640, China. Electronic address: gzcmmcjxq@1
Mater Sci Eng C Mater Biol Appl ; 98: 9-18, 2019 May.
Article in En | MEDLINE | ID: mdl-30813097
Tumor-targeted multifunctional nanocarriers play an important role in tumor diagnosis and treatment. Herein, disulfide bonds linked amphiphilic hyaluronan-SS-poly(ε-caprolactone) diblock copolymers (HA-SS-PCL) were synthesized and studied as theranostic nanocarriers for tumor diagnosis and treatment. The chemical structure of HA-SS-PCL was confirmed by Fourier transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (1H NMR). The self-assembling behavior of the HA-SS-PCL into GSH-responsive micelles and their degradation were characterized by fluorescence spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM). Theranostic nanocarriers encapsulating doxorubicin (DOX) and superparamagnetic iron oxide (SPIO) were formed via a dialysis. In vitro drug release results suggested that the HA-SS-PCL micelles possessed reductant-triggered doxorubicin release ability, which was confirmed by 100% of DOX release from HA-SS-PCL micelles within 12 h under 10 mM of glutathione (GSH), whereas about 40% of DOX was released under non-reductive condition within 24 h. Both flow cytometry and confocal laser scanning microscopy (CLSM) analysis revealed that the HA-SS-PCL micelles loaded with DOX were internalized in HepG2 cell via a receptor mediated mechanism between hyaluronan and the CD44 receptor. Furthermore, the MTT assay and cell apoptosis analysis revealed that the DOX-loaded HA-SS-PCL micelles exhibited pronounced antitumor ability towards HepG2 cells compared with that of the reduction-insensitive HA-PCL micelles at the same DOX dosage. The r2 relaxivity value of the DOX/SPIO loaded HA-SS-PCL micelles was up to 221.2 mM-1 s-1 (Fe). Thus, the obtained HA-SS-PCL block copolymers demonstrate promising potential as tumor targeting theranostic nanocarriers in the field of tumor diagnosis and treatment.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyesters / Polymers / Theranostic Nanomedicine / Hyaluronic Acid / Neoplasms Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Mater Sci Eng C Mater Biol Appl Year: 2019 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyesters / Polymers / Theranostic Nanomedicine / Hyaluronic Acid / Neoplasms Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Mater Sci Eng C Mater Biol Appl Year: 2019 Document type: Article Affiliation country: Country of publication: