Identification of Burkholderia pseudomallei by Use of the Vitek Mass Spectrometer.
J Clin Microbiol
; 57(5)2019 05.
Article
in En
| MEDLINE
| ID: mdl-30842230
ABSTRACT
Burkholderia pseudomallei is the causative agent of melioidosis. This condition most often presents as pneumonia and bacteremia, with mortality rates of 9% to 70%. Therefore, early identification of this organism may aid in directing appropriate management. This study aimed to use the Vitek matrix-assisted laser desorption ionization-time of flight mass spectrometer to create a spectrum for the rapid identification of B. pseudomallei Spectra from 85 isolate cultures were acquired using the Vitek mass spectrometer research mode. A SuperSpectrum was created using peak matching and subsequently activated for analysis of organism identification. All 85 isolates were correctly identified as B. pseudomallei A total of 899 spectra were analyzed and demonstrated a specificity of 99.8%. Eighty-one clinical isolates were used, of which 10 were neuromelioidosis, and no discernible spectrum difference was appreciated. Spectrum acquisition from a single spot was only successful in 374/899 (41%) of isolates. This increased to 100% when 3 spots of the same extract were analyzed. The Vitek mass spectrometer can be used for the rapid identification of B. pseudomallei with a high level of specificity.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Burkholderia pseudomallei
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Melioidosis
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Clin Microbiol
Year:
2019
Document type:
Article
Affiliation country: