Effect of bone sialoprotein coating on progression of bone formation in a femoral defect model in rats.
Eur J Trauma Emerg Surg
; 46(2): 277-286, 2020 Apr.
Article
in En
| MEDLINE
| ID: mdl-31139842
ABSTRACT
PURPOSE:
In orthopedic and trauma surgery, calcium phosphate cement (CPC) scaffolds are widely used as substitute for autologous bone grafts. The purpose of this study was to evaluate bone formation in a femoral condyle defect model in rats after scaffold-coating with bioactive bone sialoprotein (BSP). Our hypothesis was that BSP-coating results in additional bone formation.METHODS:
In 20 Wistar rats, defects of 3.0 mm diameter were drilled into the lateral femoral condyles of both legs. BSP-coated scaffolds or uncoated control scaffolds were implanted into the defects. After 4 and 8 weeks, five rats of each group were euthanized, respectively. µCT scans and histological analyses were performed. The ratio of bone volume-total volume (BV/TV) was analyzed and histological sections were evaluated.RESULTS:
At week four, bone fraction reached 5.2 ± 1.7% in BSP-coated scaffolds and 4.5 ± 3.2% in the control (p = 0.06). While bone fraction of the BSP-group did not change much between week four and eight [week eight 5.4 ± 3.8% (p = 0.53)], there was a tendency towards an increase in the control [week eight 7.0 ± 2.2% (p = 0.08)]. No significant difference in bone fraction were observable between BSP-coated and uncoated scaffolds at week eight (p = 0.08).CONCLUSIONS:
A significant superiority of BSP-coated scaffolds over uncoated scaffolds could not be proven. However, BSP-coating showed a tendency towards improving bone ingrowth in the scaffolds 4 weeks after implantation. This effect was only short-lived bone growth in the control scaffolds tended to outpace that of the BSP-group at week eight.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteogenesis
/
Coated Materials, Biocompatible
/
Tissue Scaffolds
/
Femur
/
Integrin-Binding Sialoprotein
Limits:
Animals
Language:
En
Journal:
Eur J Trauma Emerg Surg
Year:
2020
Document type:
Article
Affiliation country: