Screening, partial purification of antivibriosis metabolite sterol-glycosides from Rhodococcus sp. against aquaculture associated pathogens.
Microb Pathog
; 134: 103597, 2019 Sep.
Article
in En
| MEDLINE
| ID: mdl-31201903
ABSTRACT
The present study probed the antimicrobial potential of a rare mangrove associated actinomycetes against an array of aquatic bacterial pathogens causing disease outbreak in fin and shellfish. Antibacterial activity results implied that the mangrove associated actinomycetes RAS7 exhibited striking inhibitory activity against the tested aquatic bacterial pathogens. Identification of strain RAS7 through polyphasic and 16S rRNA sequencing affirmed that the strain belongs to Rhodococcus sp. Optimization of culture conditions for antibacterial activity by Rhodococcus sp. inferred that it grew well and exerted notable antagonistic activity in medium supplied with 1% galactose and peptone as carbon and nitrogen sources. Similarly, the strain grown in 0.1% tyrosine, 1% NaCl, pH 7.5 and temperature 35⯰C recorded maximum bioactivity against the test pathogens. The crude ethyl acetate extract of Rhodococcus sp. at 200 ⯵g/ml recorded markedly pronounced growth inhibitory activity ranged between 14 and 29â¯mm. The cytotoxic effect of crude extract against brine shrimp Artemia salina nauplii registered LC50 value of 134.294⯵g/ml after 24â¯h of exposure. The secondary metabolite was separated using Ethyl acetate Methanol (73) as solvent system through TLC. The TLC autobiogram mapped the active spot in TLC with Rf value of 0.84. Analysis of chemical constituents and FT-IR spectral analysis substantiated that the active principle in bioassay guided fraction was sterol-glycosides.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sterols
/
Vibrio Infections
/
Rhodococcus
/
Glycosides
/
Anti-Bacterial Agents
Type of study:
Diagnostic_studies
/
Risk_factors_studies
/
Screening_studies
Limits:
Animals
Language:
En
Journal:
Microb Pathog
Journal subject:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Year:
2019
Document type:
Article
Affiliation country: