B cells from young and old mice switch isotypes with equal frequencies after ex vivo stimulation.
Cell Immunol
; 345: 103966, 2019 Nov.
Article
in En
| MEDLINE
| ID: mdl-31447053
ABSTRACT
To determine whether old B cells have the same capacity to switch isotypes as young cells, we purified splenic follicular, marginal zone, and age-associated B cell subsets from C57BL/6 mice. Cells were stimulated in culture with interleukin 4 and either lipopolysaccharide or anti-CD40, and switching to IgG1 was measured by flow cytometry of surface immunoglobulin. The results show that switching was robust in follicular and marginal zone B cells from old mice and was comparable to their young counterparts. However, age-associated B cells from old mice switched poorly relative to the other subsets. Expression of activation-induced deaminase, which initiates switching, was quantified by qPCR of mRNA, and it was equal between young and old follicular B cells. Thus, in this ex vivo system, the follicular and marginal zone cells from young and old mice behaved similarly, showing that the molecular machinery to perform switching is intact in old B cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spleen
/
B-Lymphocytes
/
B-Lymphocyte Subsets
/
Immunoglobulin Class Switching
Limits:
Animals
Language:
En
Journal:
Cell Immunol
Year:
2019
Document type:
Article
Affiliation country: