Dissecting dynamic expression of autophagy-related genes during human fetal digestive tract development via single-cell RNA sequencing.
Autophagy
; 15(11): 2019-2021, 2019 11.
Article
in En
| MEDLINE
| ID: mdl-31470757
ABSTRACT
Macroautophagy/autophagy has been demonstrated to play an essential role in embryonic development. However, the role of autophagy during human fetal digestive tract development has not been investigated. Here, by using over 5,000 human embryonic digestive tract cells ranging from 6 weeks to 25 weeks, we explored the dynamic expression of autophagy-related genes at single-cell resolution, and found that the transcriptional activity of autophagy-related genes boosted remarkably and specifically in the early (between 6 and 9 weeks) stages. Interestingly, the small intestine cells at 9 weeks showed the most significant enrichment of autophagy-related genes than any other stages. In summary, our results for the first time revealed that autophagy may play an essential role in the development of the digestive tract, especially for the small intestine, in early human embryos. Abbreviations GI gastrointestinal; S-Intes small intestine; t-SNE t-distributed stochastic neighbor embedding.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Autophagy
/
Gastrointestinal Tract
/
Autophagy-Related Proteins
Limits:
Humans
Language:
En
Journal:
Autophagy
Year:
2019
Document type:
Article
Affiliation country: