Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling.
Chem Commun (Camb)
; 55(76): 11414-11417, 2019 Sep 19.
Article
in En
| MEDLINE
| ID: mdl-31482872
ABSTRACT
Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enabling simultaneous quantification of the lesions on individual DNA molecules. We follow the repair of both damage types in skin cells exposed to artificial sunlight.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ultraviolet Rays
/
DNA Damage
/
DNA
/
Color
/
Fluorescent Dyes
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2019
Document type:
Article
Affiliation country: