Production and Thermal Exchange of Conditional Peptide-MHC I Multimers.
Curr Protoc Immunol
; 126(1): e85, 2019 09.
Article
in En
| MEDLINE
| ID: mdl-31483102
ABSTRACT
Cytotoxic CD8+ T cells mediate cellular immunity through recognition of specific antigens presented by MHC class I on all nucleated cells. Studying T cell interactions and responses provides invaluable information on infection, autoimmunity and cancer. Fluorescently labeled multimers of MHC I can be used to quantify, characterize, and isolate specific CD8+ T cells by flow cytometry. Here we describe the production and use of conditional MHC I multimers that can be loaded with peptides of choice by incubating them at a defined temperature. Multimers are folded with a template peptide that forms a stable complex at low temperature, but dissociates at a defined elevated temperature. Using this technology multiple MHC I multimers can be generated in parallel, to allow staining and isolation of large sets of antigen-specific CD8+ T cells, especially when combined with barcoding technologies. © 2019 The Authors.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Staining and Labeling
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T-Lymphocytes, Cytotoxic
/
Histocompatibility Antigens Class I
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Cell Separation
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CD8-Positive T-Lymphocytes
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Antigens
Limits:
Animals
/
Humans
Language:
En
Journal:
Curr Protoc Immunol
Year:
2019
Document type:
Article
Affiliation country: