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Mitochondrial DNA: A Biomarker of Disease Severity in Necrotizing Enterocolitis.
Bindi, Edoardo; Li, Bo; Zhou, Haitao; Janssen Lok, Maarten; Alganabi, Mashriq; Angotti, Rossella; Pierro, Agostino.
Affiliation
  • Bindi E; Division of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, Ontario, Canada.
  • Li B; Division of Pediatric Surgery, Department of Medical Sciences, Surgical Sciences and Neurosciences, Hospital of "Santa Maria alle Scotte," Siena, Italy.
  • Zhou H; Division of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, Ontario, Canada.
  • Janssen Lok M; Division of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, Ontario, Canada.
  • Alganabi M; Division of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, Ontario, Canada.
  • Angotti R; Division of General and Thoracic Surgery, Translational Medicine Program, The Hospital for Sick Children, Toronto, Ontario, Canada.
  • Pierro A; Division of Pediatric Surgery, Department of Medical Sciences, Surgical Sciences and Neurosciences, Hospital of "Santa Maria alle Scotte," Siena, Italy.
Eur J Pediatr Surg ; 30(1): 85-89, 2020 Feb.
Article in En | MEDLINE | ID: mdl-31600798
INTRODUCTION: There is a need to develop sensitive markers to diagnose or monitor the severity of intestinal damage in necrotizing enterocolitis (NEC). Mitochondrial deoxyribonucleic acid (mtDNA) is increased in the intestine and blood of adults in response to intestinal ischemia and can trigger secondary organ damage. We hypothesize that mtDNA is increased during experimental NEC and that mtDNA levels are correlated to the degree of intestinal injury. MATERIALS AND METHODS: NEC was induced in C57BL/6 mice (n = 18) (approval: 44032) by gavage feeding with hyperosmolar formula, hypoxia, and lipopolysaccharide administration from postnatal day (P) 5 to 9. Breastfed pups served as control (n = 15). Blood was collected by cardiac puncture and terminal ileum was harvested on P9. Reverse transcription quatitative polymerase chain reaction was used to measure mtDNA (markers COX3, CYTB, ND1) and inflammatory cytokines (interleukin 6 [IL-6] and tumor necrosis factor-α[TNF-α]) in blood and ileum. Intestinal injury was scored blindly by four investigators and classified as no/minor injury (score 0 or 1) or NEC (score ≥2). RESULTS: mtDNA is significantly increased in gut and blood of NEC mice (p < 0.05). Furthermore, mtDNA increases in intestine and blood proportionally to the degree of intestinal injury as indicated by a positive correlation with histological scoring and inflammation (r = 0.6; p < 0.05) (expression of IL-6 and TNF-α). CONCLUSION: Following NEC intestinal injury, mtDNA is released from the intestine into circulation. The blood level of mtDNA is related to the degree of intestinal injury. mtDNA can be a novel marker of intestinal injury and can be useful for monitoring the progression of NEC.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Mitochondrial / Enterocolitis, Necrotizing Type of study: Prognostic_studies Limits: Animals Language: En Journal: Eur J Pediatr Surg Journal subject: PEDIATRIA Year: 2020 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Mitochondrial / Enterocolitis, Necrotizing Type of study: Prognostic_studies Limits: Animals Language: En Journal: Eur J Pediatr Surg Journal subject: PEDIATRIA Year: 2020 Document type: Article Affiliation country: Country of publication: