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Riboflavin Supplementation in Patients with Crohn's Disease [the RISE-UP study].
von Martels, Julius Z H; Bourgonje, Arno R; Klaassen, Marjolein A Y; Alkhalifah, Hassan A A; Sadaghian Sadabad, Mehdi; Vich Vila, Arnau; Gacesa, Ranko; Gabriëls, Ruben Y; Steinert, Robert E; Jansen, Bernadien H; Bulthuis, Marian L C; van Dullemen, Hendrik M; Visschedijk, Marijn C; Festen, Eleonora A M; Weersma, Rinse K; de Vos, Paul; van Goor, Harry; Faber, Klaas Nico; Harmsen, Hermie J M; Dijkstra, Gerard.
Affiliation
  • von Martels JZH; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Bourgonje AR; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Klaassen MAY; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Alkhalifah HAA; Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Sadaghian Sadabad M; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Vich Vila A; Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Gacesa R; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Gabriëls RY; Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Steinert RE; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Jansen BH; Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Bulthuis MLC; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • van Dullemen HM; R&D Human Nutrition and Health, DSM Nutritional Products Ltd, Basel, Switzerland.
  • Visschedijk MC; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Festen EAM; Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Weersma RK; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • de Vos P; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • van Goor H; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Faber KN; Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Harmsen HJM; Department of Gastroenterology and Hepatology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
  • Dijkstra G; Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
J Crohns Colitis ; 14(5): 595-607, 2020 Jun 19.
Article in En | MEDLINE | ID: mdl-31873717
ABSTRACT
BACKGROUND AND

AIMS:

Crohn's disease [CD] is characterised by chronic intestinal inflammation and dysbiosis in the gut. Riboflavin [vitamin B2] has anti-inflammatory, antioxidant and microbiome-modulatory properties. Here, we analysed the effect of riboflavin on oxidative stress, markers of inflammation, clinical symptoms, and faecal microbiome in patients with CD.

METHODS:

In this prospective clinical intervention study, patients received 100 mg riboflavin [DSM, Nutritional Products Ltd] daily for 3 weeks. Clinical disease activity [Harvey-Bradshaw Index HBI], serum biomarkers of inflammation and redox status [plasma free thiols], and faecal microbiome taxonomical composition and functionality [fluorescent in situ hybridisation FISH; and metagenomic shotgun sequencing MGS], were analysed before and after riboflavin intervention.

RESULTS:

In total, 70 patients with CD with varying disease activity were included. Riboflavin supplementation significantly decreased serum levels of inflammatory markers. In patients with low faecal calprotectin [FC] levels, IL-2 decreased, and in patients with high FC levels, C-reactive protein [CRP] was reduced and free thiols significantly increased after supplementation. Moreover, HBI was significantly decreased by riboflavin supplementation. Riboflavin supplementation led to decreased Enterobacteriaceae in patients with low FC levels as determined by FISH; however, MGS analysis showed no effects on diversity, taxonomy, or metabolic pathways of the faecal microbiome.

CONCLUSIONS:

Three weeks of riboflavin supplementation resulted in a reduction in systemic oxidative stress, mixed anti-inflammatory effects, and a reduction in clinical symptoms [HBI]. FISH analysis showed decreased Enterobacteriaceae in patients with CD with low FC levels, though this was not observed in MGS analysis. Our data demonstrate that riboflavin supplementation has a number of anti-inflammatory and anti-oxidant effects in CD.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Riboflavin / Vitamin B Complex / Crohn Disease / Oxidative Stress / Gastrointestinal Microbiome Type of study: Observational_studies Aspects: Patient_preference Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: J Crohns Colitis Journal subject: GASTROENTEROLOGIA Year: 2020 Document type: Article Affiliation country:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Riboflavin / Vitamin B Complex / Crohn Disease / Oxidative Stress / Gastrointestinal Microbiome Type of study: Observational_studies Aspects: Patient_preference Limits: Adult / Female / Humans / Male / Middle aged Language: En Journal: J Crohns Colitis Journal subject: GASTROENTEROLOGIA Year: 2020 Document type: Article Affiliation country: