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USP27X negatively regulates antiviral signaling by deubiquitinating RIG-I.
Tao, Xinyue; Chu, Bei; Xin, Di; Li, Lin; Sun, Qinmiao.
Affiliation
  • Tao X; State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • Chu B; Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China.
  • Xin D; State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
  • Li L; School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.
  • Sun Q; State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
PLoS Pathog ; 16(2): e1008293, 2020 02.
Article in En | MEDLINE | ID: mdl-32027733
RIG-I plays important roles in pathogen sensing and activation of antiviral innate immune responses in response to RNA viruses. RIG-I-mediated signaling must be precisely controlled to maintain innate immune signaling homeostasis. Previous studies demonstrated that lysine 63 (K63)-linked polyubiquitination of RIG-I is vital for its activation, but the mechanisms through which RIG-I is deubiquitinated to control innate immune responses are not well understood. Here we identified USP27X as a negative regulator of antiviral signaling in response to RNA viruses through siRNA library screening. Further functional studies indicated that USP27X negatively modulated RIG-I-mediated antiviral signaling in a deubiquitinase-dependent manner. Mechanistically, we found that USP27X removed K63-linked polyubiquitin chains from RIG-I to negatively modulate type I interferon signaling. Collectively, these studies uncover a novel negative regulatory role of USP27X in targeting RIG-I to balance innate immune responses.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viruses / Signal Transduction / Ubiquitin-Specific Proteases / DEAD Box Protein 58 / Immunity, Innate Limits: Animals / Humans Language: En Journal: PLoS Pathog Year: 2020 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viruses / Signal Transduction / Ubiquitin-Specific Proteases / DEAD Box Protein 58 / Immunity, Innate Limits: Animals / Humans Language: En Journal: PLoS Pathog Year: 2020 Document type: Article Affiliation country: Country of publication: