The Shift of HbF to HbA under Influence of SKA2 Gene; A Possible Link between Cortisol and Hematopoietic Maturation in Term and Preterm Newborns.
Endocr Metab Immune Disord Drug Targets
; 21(3): 485-494, 2021.
Article
in En
| MEDLINE
| ID: mdl-32364083
ABSTRACT
BACKGROUND:
We hypothesized that the SKA2 gene can convert hemoglobin F to A leading to the maturity of the hematopoietic system by glucocorticoid hormone; so, the present study aimed to investigate the health outcome of newborns by using the effect of SKA2 gene on hematopoietic maturation.METHODS:
At first, 142 samples were divided into term and preterm. After sampling from the umbilical cord blood, the expression of SKA2 genes and HbA and F were evaluated by quantitative RT-PCR. The blood gases were measured by Campact 3 device. Finally, the cortisol level was measured by ELISA method and HbA and F levels were investigated by capillary electrophoresis.RESULTS:
The blood gases and Apgar scores were more favorable in term newborns (P <0.001). Levels of protein/expression of HbF in newborns with Apgar score greater than 7 was lower than that of the newborns with Apgar score below 7 (P <0.001). Cortisol and HbA levels were considerably higher in term newborns compared to the preterm ones (P <0.001). In the preterm and term groups, SKA2 gene expression had a positive and significant relationship with cortisol and HbA levels as well as a negative relationship with the HbF level. In the preterm group, a positive and significant relationship was observed between the expression of SKA2 and HbF genes.CONCLUSION:
The results revealed that the SKA2 gene affected hematopoietic maturation in preterm and term newborns and the health outcome of newborns improved by increasing HbA level.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fetal Hemoglobin
/
Hemoglobin A
/
Infant, Premature
/
Hematopoietic Stem Cells
/
Hydrocortisone
/
Chromosomal Proteins, Non-Histone
/
Hematopoiesis
Limits:
Humans
/
Newborn
Language:
En
Journal:
Endocr Metab Immune Disord Drug Targets
Journal subject:
ALERGIA E IMUNOLOGIA
/
ENDOCRINOLOGIA
/
METABOLISMO
/
TERAPIA POR MEDICAMENTOS
Year:
2021
Document type:
Article
Affiliation country: