Nanoadhesive layer to prevent protein absorption in a poly(dimethylsiloxane) microfluidic device.
Biotechniques
; 69(1): 404-409, 2020 07.
Article
in En
| MEDLINE
| ID: mdl-32372656
ABSTRACT
Poly(dimethylsiloxane) (PDMS) is widely used as a microfluidics platform material; however, it absorbs various molecules, perturbing specific chemical concentrations in microfluidic channels. We present a simple solution to prevent adsorption into a PDMS microfluidic device. We used a vapor-phase-deposited nanoadhesive layer to seal PDMS microfluidic channels. Absorption of fluorescent molecules into PDMS was efficiently prevented in the nanolayer-treated PDMS device. Importantly, when cultured in a nanolayer-treated PDMS device, yeast cells exhibited the expected concentration-dependent response to a mating pheromone, including mating-specific morphological and gene expression changes, while yeast cultured in an untreated PDMS device did not properly respond to the pheromone. Our method greatly expands microfluidic applications that require precise control of molecule concentrations.
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Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Biotechniques
Year:
2020
Document type:
Article
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