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Mycolicibacterium smegmatis possesses operational agmatinase but contains no detectable polyamines.
Zamakhaev, Mikhail; Tsyganov, Ivan; Nesterova, Larisa; Akhova, Anna; Grigorov, Artem; Bespyatykh, Julia; Azhikina, Tatyana; Tkachenko, Alexander; Shumkov, Mikhail.
Affiliation
  • Zamakhaev M; Research Center of Biotechnology of the Russian Academy of Sciences, Bach Institute of Biochemistry, Moscow, Russia.
  • Tsyganov I; Perm State University, Biology Faculty; Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Institute of Ecology and Genetics of Microorganisms, Perm, Russia.
  • Nesterova L; Perm State University, Biology Faculty; Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Institute of Ecology and Genetics of Microorganisms, Perm, Russia.
  • Akhova A; Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Institute of Ecology and Genetics of Microorganisms, Perm, Russia.
  • Grigorov A; Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Department of Genomics and Postgenomic Technologies, Moscow, Russia.
  • Bespyatykh J; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.
  • Azhikina T; Shemyakin and Ovchinnikov, Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Department of Genomics and Postgenomic Technologies, Moscow, Russia.
  • Tkachenko A; Perm State University, Biology Faculty; Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Institute of Ecology and Genetics of Microorganisms, Perm, Russia.
  • Shumkov M; Research Center of Biotechnology of the Russian Academy of Sciences, Bach Institute of Biochemistry, Moscow, Russia.
Int J Mycobacteriol ; 9(2): 138-143, 2020.
Article in En | MEDLINE | ID: mdl-32474534
ABSTRACT

Background:

Polyamines are widespread intracellular molecules able to influence antibiotic susceptibility, but almost nothing is known on their occurrence and physiological role in mycobacteria.

Methods:

here, we analyzed transcriptomic, proteomic and biochemical data and obtained the first evidence for the post-transcriptional expression of some genes attributed to polyamine metabolism and polyamine transport in Mycolicibacterium smegmatis (basionym Mycobacterium smegmatis).

Results:

in our experiments, exponentially growing cells demonstrated transcription of 21 polyamine-associated genes and possessed 7 enzymes of polyamine metabolism and 2 polyamine transport proteins.

Conclusion:

Mycolicibacterium smegmatis putrescine synthesizing enzyme agmatinase SpeB was originally shown to catalyze agmatine conversion to putrescine in vitro. Nevertheless, we have not found any polyamines in mycobacterial cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyamines / Ureohydrolases / Mycobacterium smegmatis Language: En Journal: Int J Mycobacteriol Year: 2020 Document type: Article Affiliation country:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyamines / Ureohydrolases / Mycobacterium smegmatis Language: En Journal: Int J Mycobacteriol Year: 2020 Document type: Article Affiliation country: