Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System.
J Vis Exp
; (159)2020 05 22.
Article
in En
| MEDLINE
| ID: mdl-32510501
ABSTRACT
Protein interactions at cellular interfaces dictate a multitude of biological outcomes ranging from tissue development and cancer progression to synapse formation and maintenance. Many of these fundamental interactions occur in trans and are typically induced by heterophilic or homophilic interactions between cells expressing membrane anchored binding pairs. Elucidating how disease relevant mutations disrupt these fundamental protein interactions can provide insight into a myriad of cell biology fields. Many protein-protein interaction assays do not typically disambiguate between cis and trans interactions, which potentially leads to an overestimation of the extent of binding that is occurring in vivo and involve labor intensive purification of protein and/or specialized monitoring equipment. Here, we present an optimized simple protocol that allows for the observation and quantification of only trans interactions without the need for lengthy protein purifications or specialized equipment. The HEK cell aggregation assay involves the mixing of two independent populations of HEK cells, each expressing membrane-bound cognate ligands. After a short incubation period, samples are imaged and the resulting aggregates are quantified.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Communication
/
Cytological Techniques
/
Protein Aggregates
Limits:
Humans
Language:
En
Journal:
J Vis Exp
Year:
2020
Document type:
Article