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Dihydrotestosterone (DHT) Enhances Wound Healing of Major Burn Injury by Accelerating Resolution of Inflammation in Mice.
Shi, Huaikai; Lo, Tsun-Ho; Ma, Duncan; Condor, Brenton; Lesmana, Brian; Parungao, Roxanne J; Tsai, Kevin H-Y; Kim, Sarah; Chen, Hsiao-Ting; Silveira, Pablo A; Li, Zhe; Cooper, Mark S; Simanainen, Ulla; Handelsman, David J; Maitz, Peter K; Wang, Yiwei.
Affiliation
  • Shi H; Burns Research and Reconstructive Surgery, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Lo TH; Dendritic Cell Research, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Ma D; Burns Research and Reconstructive Surgery, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Condor B; Burns Research and Reconstructive Surgery, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Lesmana B; Burns Research and Reconstructive Surgery, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Parungao RJ; Burns Research and Reconstructive Surgery, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Tsai KH; Adrenal Steroids Laboratory, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Kim S; Bone Biology Group, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Chen HT; Dendritic Cell Research, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Silveira PA; Dendritic Cell Research, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Li Z; Burns Research and Reconstructive Surgery, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Cooper MS; Burns Unit, Concord Repatriation General Hospital, Sydney 2139, Australia.
  • Simanainen U; Adrenal Steroids Laboratory, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Handelsman DJ; Andrology Laboratory, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Maitz PK; Andrology Laboratory, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
  • Wang Y; Burns Research and Reconstructive Surgery, ANZAC Research Institute, University of Sydney, Sydney 2139, Australia.
Int J Mol Sci ; 21(17)2020 Aug 28.
Article in En | MEDLINE | ID: mdl-32872240
Androgens have been known to inhibit cutaneous wound healing in men and male mice. However, in children with major burn injuries, a synthetic androgen was reported clinically to improve wound healing. The aim of this study is to investigate the role of dihydrotestosterone (DHT) as a new therapeutic approach in treating major burn injury. In the present study, mice received systemic androgen treatment post major burn injury. Wound healing rate and body weight were monitored over 21 days. The serum level of inflammatory cytokines/chemokines were measured using multiplex immunoassays. In addition, splenocyte enumeration was performed by flow cytometry. Healing phases of inflammation, re-epithelialization, cell proliferation and collagen deposition were also examined. In results, DHT treated mice lost less weight and displayed accelerated wound healing but has no impact on hypermetabolism. Mice, after burn injury, displayed acute systemic inflammatory responses over 21 days. DHT treatment shortened the systemic inflammatory response with reduced splenic weight and monocyte numbers on day 14 and 21. DHT treatment also reduced wound infiltrating macrophage numbers. In conclusion, DHT treatment facilitates local wound healing by accelerating the resolution of inflammation, but not through alterations of post-burn hypermetabolic response.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Dihydrotestosterone / Wound Healing / Burns / Androgens Limits: Animals Language: En Journal: Int J Mol Sci Year: 2020 Document type: Article Affiliation country: Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Dihydrotestosterone / Wound Healing / Burns / Androgens Limits: Animals Language: En Journal: Int J Mol Sci Year: 2020 Document type: Article Affiliation country: Country of publication: