Flavin dependency undermines proteome stability, lipid metabolism and cellular proliferation during vitamin B2 deficiency.
Cell Death Dis
; 11(9): 725, 2020 09 07.
Article
in En
| MEDLINE
| ID: mdl-32895367
ABSTRACT
Tumor cells adapt their metabolism to meet the energetic and anabolic requirements of high proliferation and invasiveness. The metabolic addiction has motivated the development of therapies directed at individual biochemical nodes. However, currently there are few possibilities to target multiple enzymes in tumors simultaneously. Flavin-containing enzymes, ca. 100 proteins in humans, execute key biotransformations in mammalian cells. To expose metabolic addiction, we inactivated a substantial fraction of the flavoproteome in melanoma cells by restricting the supply of the FMN and FAD precursor riboflavin, the vitamin B2. Vitamin B2 deficiency affected stability of many polypeptides and thus resembled the chaperone HSP90 inhibition, the paradigmatic multiple-target approach. In support of this analogy, flavin-depleted proteins increasingly associated with a number of proteostasis network components, as identified by the mass spectrometry analysis of the FAD-free NQO1 aggregates. Proteome-wide analysis of the riboflavin-starved cells revealed a profound inactivation of the mevalonate pathway of cholesterol synthesis, which underlines the manifold cellular vulnerability created by the flavoproteome inactivation. Cell cycle-arrested tumor cells became highly sensitive to alkylating chemotherapy. Our data suggest that the flavoproteome is well suited to design synthetic lethality protocols combining proteostasis manipulation and metabolic reprogramming.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Riboflavin
/
Proteome
/
Flavin-Adenine Dinucleotide
Type of study:
Guideline
Limits:
Animals
/
Humans
Language:
En
Journal:
Cell Death Dis
Year:
2020
Document type:
Article
Affiliation country: