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SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.
Mizutani, Yusuke; Omagari, Daisuke; Hayatsu, Manabu; Nameta, Masaaki; Komiyama, Kazuo; Mikami, Yoshikazu; Ushiki, Tatsuo.
Affiliation
  • Mizutani Y; Division of Microscopic Anatomy, Niigata University Graduate School of Medical and Dental Sciences , Niigata-shi, Japan.
  • Omagari D; Office of Institutional Research, Hokkaido University , Kita-ku, Japan.
  • Hayatsu M; Department of Pathology, Nihon University School of Dentistry , Tokyo, Japan.
  • Nameta M; Division of Microscopic Anatomy, Niigata University Graduate School of Medical and Dental Sciences , Niigata-shi, Japan.
  • Komiyama K; Electron Microscope Core Facility, Niigata University , Niigata-shi, Japan.
  • Mikami Y; Department of Pathology, Nihon University School of Dentistry , Tokyo, Japan.
  • Ushiki T; Division of Microscopic Anatomy, Niigata University Graduate School of Medical and Dental Sciences , Niigata-shi, Japan.
Cell Adh Migr ; 14(1): 195-203, 2020 12.
Article in En | MEDLINE | ID: mdl-33016205
To elucidate the underlying mechanism of secretory leukocyte protease inhibitor (SLPI)-induced cell migration, we compared SLPI-deleted human gingival carcinoma Ca9-22 (ΔSLPI) cells and original (wild-type: wt) Ca9-22 cells using several microscopic imaging methods and gene expression analysis. Our results indicated reduced migration of ΔSLPI cells compared to wtCa9-22 cells. The lamellipodia/dorsal ruffles were smaller and moved slower in ΔSLPI cells compared to wtCa9-22 cells. Furthermore, well-developed intermediate filament bundles were observed at the desmosome junction of ΔSLPI cells. In addition, Galectin4 was strongly expressed in ΔSLPI cells, and its forced expression suppressed migration of wtCa9-22 cells. Taken together, SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pseudopodia / Cell Movement / Galectin 4 / Desmosomes / Secretory Leukocyte Peptidase Inhibitor Limits: Humans Language: En Journal: Cell Adh Migr Year: 2020 Document type: Article Affiliation country: Country of publication:
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pseudopodia / Cell Movement / Galectin 4 / Desmosomes / Secretory Leukocyte Peptidase Inhibitor Limits: Humans Language: En Journal: Cell Adh Migr Year: 2020 Document type: Article Affiliation country: Country of publication: