A Functional Chemiluminescent Probe for in Vivo Imaging of Natural Killer Cell Activity Against Tumours.
Angew Chem Int Ed Engl
; 60(11): 5699-5703, 2021 03 08.
Article
in En
| MEDLINE
| ID: mdl-33300671
ABSTRACT
Natural killer (NK) cells are immune cells that can kill certain types of cancer cells. Adoptive transfer of NK cells represents a promising immunotherapy for malignant tumours; however, there is a lack of methods to validate anti-tumour activity of NK cells inâ
vivo. Herein, we report a new chemiluminescent probe to image inâ
situ the granzymeâ
B-mediated killing activity of NK cells against cancer cells. We have optimised a granzymeâ
B-specific construct using an activatable phenoxydioxetane reporter so that enzymatic cleavage of the probe results in bright chemiluminescence. The probe shows high selectivity for active granzymeâ
B over other proteases and higher signal-to-noise ratios than commercial fluorophores. Finally, we demonstrate that the probe can detect NK cell activity in mouse models, being the first chemiluminescent probe for inâ
vivo imaging of NK cell activity in live tumours.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Killer Cells, Natural
/
Granzymes
/
Fluorescent Dyes
/
Neoplasms
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Angew Chem Int Ed Engl
Year:
2021
Document type:
Article
Affiliation country: